直肠癌患者MDSC百分比和RORα mRNA、IL-17E mRNA、FOXP3 mRNA表达的临床意义

doi:10.3969/j.issn.1673-8640.2023.01.005

摘要/Abstract

摘要：

目的探讨外周血单个核细胞（PBMC）中视黄酸相关孤儿核受体α（RORα） mRNA、白细胞介素17E（IL-17E） mRNA、叉头框蛋白 P3（FOXP3） mRNA和髓源性抑制细胞（MDSC）百分比在直肠癌中的临床价值。方法选取直肠癌患者95例（直肠癌组）、健康体检者45名（正常对照组），收集所有研究对象的临床资料，检测所有研究对象PBMC中RORα mRNA、FOXP3 mRNA、IL-17E mRNA水平和MDSC百分比。结果直肠癌组FOXP3 mRNA、IL-17E mRNA相对表达量和MDSC百分比均高于正常对照组（P<0.05），RORα mRNA相对表达量低于正常对照组（P<0.05）。有淋巴转移、TNM分期Ⅲ期的直肠癌患者FOXP3 mRNA、IL-17E mRNA相对表达量和MDSC百分比分别高于无淋巴转移、TNM分期Ⅰ~Ⅱ期的患者（P<0.05），RORα mRNA相对表达量分别低于无淋巴转移、TNM分期Ⅰ~Ⅱ期的患者（P<0.05）。不同性别、年龄、肿瘤直径的直肠癌患者之间RORα mRNA、FOXP3 mRNA、IL-17E mRNA相对表达量和MDSC百分比差异均无统计学意义（P>0.05）。结论直肠癌患者IL-17E mRNA、FOXP3 mRNA水平和MDSC显著升高，RORα mRNA水平显著降低，且与TNM分期和淋巴转移有关，或可作为直肠癌筛查和病情监测的指标。

Abstract:

Objective To investigate the retinoid-related orphan receptor alpha（RORα） mRNA，interleukin-17E（IL-17E） mRNA and forkhead box protein P3（FOXP3） mRNA in peripheral blood mononuclear cells（PBMC） and myelogenous suppressor cell（MDSC） percentage of patients with rectal cancer. Methods The clinical data of 95 patients with rectal cancer（rectal cancer group） and 45 healthy subjects（healthy control group） were collected，and the expressions of RORα mRNA，FOXP3 mRNA and IL-17E mRNA in PBMC and MDSC percentage were determined. Results The relative expression levels of FOXP3 mRNA，IL-17E mRNA and MDSC percentage in rectal cancer group were higher than those in healthy control group（P<0.05），and the relative expression level of RORα mRNA was lower than that in healthy control group（P<0.05）. The relative expression levels of FOXP3 mRNA，IL-17E mRNA and MDSC percentage in rectal cancer patients with lymph metastasis and TNM stage Ⅲ were higher than those in patients without lymph metastasis and TNM stage Ⅰ-Ⅱ（P<0.05），and the relative expression level of RORα mRNA was lower than that in patients without lymph metastasis and TNM stage Ⅰ-Ⅱ（P<0.05）. There was no statistical significance in RORα mRNA，FOXP3 mRNA，IL-17E mRNA relative expression levels and MDSC percentage in rectal cancer patients with different sex，ages and tumor diameters（P>0.05）. Conclusions IL-17E mRNA and FOXP3 mRNA expressions and MDSC percentage are increased，and RORα mRNA expression is decreased in patients with rectal cancer. These indicators are related to TNM stage and lymph metastasis，and they may be used as screening and disease monitoring indicators for rectal cancer.

Key words: Retinoid-related orphan receptor alpha, Interleukin-17E, Forkhead box protein P3, Myeloid-derived suppressor cell, Peripheral blood mononuclear cell, Rectal cancer

中图分类号:

R446.1

杨艳敏, 彭梦乐, 连文萍. 直肠癌患者MDSC百分比和RORα mRNA、IL-17E mRNA、FOXP3 mRNA表达的临床意义[J]. 检验医学, 2023, 38(1): 23-27.

YANG Yanmin, PENG MengLe, LIAN Wenping. MDSC percentage and the expressions of RORα mRNA，IL-17E mRNA and FOXP3 mRNA in rectal cancer patients[J]. Laboratory Medicine, 2023, 38(1): 23-27.

图/表 2

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组别	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
直肠癌组	95	0.56±0.15	1.76±0.51	1.63±0.46	2.63±0.76
正常对照组	45	1.13±0.27	1.03±0.32	0.93±0.26	1.53±0.46
t值		16.038	8.807	9.503	8.953
P值		0.000	0.000	0.000	0.000

组别	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
直肠癌组	95	0.56±0.15	1.76±0.51	1.63±0.46	2.63±0.76
正常对照组	45	1.13±0.27	1.03±0.32	0.93±0.26	1.53±0.46
t值		16.038	8.807	9.503	8.953
P值		0.000	0.000	0.000	0.000

项目	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
性别
女	52	0.52±0.12	1.71±0.48	1.64±0.45	2.63±0.72
男	43	0.55±0.17	1.74±0.51	1.61±0.40	2.59±0.70
t值		1.006	0.295	0.340	0.273
P值		0.317	0.769	0.735	0.786
年龄
<50岁	35	0.55±0.14	1.73±0.50	1.60±0.39	2.58±0.67
≥50岁	60	0.58±0.16	1.70±0.49	1.63±0.42	2.65±0.74
t值		0.922	0.286	0.345	0.460
P值		0.359	0.776	0.731	0.646
肿瘤直径
<5 cm	55	0.56±0.14	1.68±0.47	1.58±0.36	2.53±0.62
≥5 cm	40	0.51±0.12	1.65±0.46	1.55±0.33	2.59±0.66
t值		1.823	0.310	0.415	0.453
P值		0.072	0.757	0.679	0.561
项目	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
淋巴转移
有	60	0.43±0.11	2.07±0.56	1.77±0.43	2.76±0.24
无	35	0.56±0.15	1.67±0.43	1.56±0.35	2.51±0.20
t值		4.876	3.778	2.537	5.369
P值		0.000	0.000	0.013	0.000
TNM 分期
Ⅰ~Ⅱ期	62	0.54±0.14	1.70±0.49	1.57±0.36	2.53±0.32
Ⅲ期	33	0.40±0.09	2.10±0.57	1.76±0.42	2.77±0.39
t值		4.980	3.577	2.310	3.222
P值		0.000	0.000	0.023	0.002

项目	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
性别
女	52	0.52±0.12	1.71±0.48	1.64±0.45	2.63±0.72
男	43	0.55±0.17	1.74±0.51	1.61±0.40	2.59±0.70
t值		1.006	0.295	0.340	0.273
P值		0.317	0.769	0.735	0.786
年龄
<50岁	35	0.55±0.14	1.73±0.50	1.60±0.39	2.58±0.67
≥50岁	60	0.58±0.16	1.70±0.49	1.63±0.42	2.65±0.74
t值		0.922	0.286	0.345	0.460
P值		0.359	0.776	0.731	0.646
肿瘤直径
<5 cm	55	0.56±0.14	1.68±0.47	1.58±0.36	2.53±0.62
≥5 cm	40	0.51±0.12	1.65±0.46	1.55±0.33	2.59±0.66
t值		1.823	0.310	0.415	0.453
P值		0.072	0.757	0.679	0.561
项目	例数	RORα mRNA	FOXP3 mRNA	IL-17E mRNA	MDSC百分比/%
淋巴转移
有	60	0.43±0.11	2.07±0.56	1.77±0.43	2.76±0.24
无	35	0.56±0.15	1.67±0.43	1.56±0.35	2.51±0.20
t值		4.876	3.778	2.537	5.369
P值		0.000	0.000	0.013	0.000
TNM 分期
Ⅰ~Ⅱ期	62	0.54±0.14	1.70±0.49	1.57±0.36	2.53±0.32
Ⅲ期	33	0.40±0.09	2.10±0.57	1.76±0.42	2.77±0.39
t值		4.980	3.577	2.310	3.222
P值		0.000	0.000	0.023	0.002