AS患者sLAIR-1和LAIR-1 mRNA的表达及其与疾病活动度的关系

doi:10.3969/j.issn.1673-8640.2022.010.002

摘要/Abstract

摘要：

目的探讨强直性脊柱炎（AS）患者血清可溶性白细胞相关免疫球蛋白样受体-1（sLAIR-1）及外周血单个核细胞（PBMC）中白细胞相关免疫球蛋白样受体-1（LAIR-1）mRNA表达的变化，及其与AS疾病活动度的关系。方法选取AS患者81例（AS组），收集其一般资料、病史资料和入院后首次实验室检测结果，检测其血清sLAIR-1、白细胞介素（IL）-6、IL-17水平和PBMC中LAIR-1 mRNA的相对表达量。以健康体检者80名作为正常对照组。比较AS组与正常对照组各项指标的差异。检测81例AS患者中17例活动期患者英孚利昔单抗治疗6周后的sLAIR-1和LAIR-1 mRNA水平。采用Spearman相关分析评估血清sLAIR-1、LAIR-1 mRNA与各项指标的相关性。结果 AS组血清sLAIR-1、IL-17及IL-6水平显著高于正常对照组（P<0.001），LAIR-1 mRNA水平显著低于正常对照组（P<0.001）。Spearman相关分析结果显示，sLAIR-1与IL-17、IL-6、C反应蛋白（CRP）、基于CRP的强直性脊柱炎疾病活动评分（ASDAS-CRP）呈正相关（r值分别为0.482 4、0.475 3、0.391 6、0.565 7，P<0.01），与LAIR-1 mRNA呈负相关（r=-0.446 6，P<0.001）；LAIR-1 mRNA与IL-17、IL-6、CRP、ASDAS-CRP呈负相关（r值分别为-0.399 6、-0.227 8、-0.265 1、-0.366 8，P<0.05）。与治疗前比较，AS患者采用英孚利昔单抗治疗6周后，血清sLAIR-1水平显著下降，LAIR-1 mRNA水平显著升高，差异均有统计学意义（P<0.05）。结论 LAIR-1水平与AS疾病活动度密切相关，或可作为临床评估AS患者疾病活动度的辅助指标。

Abstract:

Objective To investigate the changes of serum soluble leukocyte-associated immunoglobulin-like receptor-1（LAIR-1）（sLAIR-1） and LAIR-1 mRNA in peripheral blood mononuclear cells（PBMC） of patients with ankylosing spondylitis（AS） and their relationship with disease activity. Methods Totally，81 patients with AS were enrolled as AS group，and another 80 healthy subjects were enrolled as control group. The patients' clinical data and routine laboratory determination results were recorded. Serum sLAIR-1，interleukin（IL）-6 and IL-17 levels were determined，and the relative expression of LAIR-1 mRNA was determined as well. The difference between AS and control groups was compared. LAIR-1 and LAIR-1 mRNA levels were also determined in 17 patients with active AS of the 81 AS patients after 6 weeks of infliximab treatment. The correlations between sLAIR-1 and LAIR-1mRNA and the other indicators were analyzed by Spearman correlation analysis. Results Serum sLAIR-1，IL-17 and IL-6 in AS group were higher than those in control group（P<0.001），while LAIR-1 mRNA levels in AS group were lower（P<0.001）. Spearman correlation analysis showed that sLAIR-1 was positively correlated with IL-17，IL-6 and C-reactive protein（CRP）（r values were 0.482 4，0.475 3 and 0.391 6，respectively，P<0.01） and with the ankylosing spondylitis disease activity score（ASDAS） with CRP（ASDAS-CRP）（r value was 0.565 7，P<0.01），while LAIR-1 mRNA was negatively correlated with SLAIR-1， IL-17，IL-6，CRP and ASDAS-CRP（r values were -0.446 6，-0.399 6，-0.227 8，-0.265 1 and -0.366 8，respectively，P<0.05）. Serum sLAIR-1 was decreased（P<0.05），and LAIR-1 mRNA was increased（P<0.05） in patients with active AS after 6 weeks of infliximab treatment. Conclusions LAIR-1 was closely related to the disease activity，indicating that it may be used as a new indicator for the evaluation of AS disease activity.

Key words: Leukocyte-associated immunoglobulin-like receptor-1, Inflammatory cytokine, Peripheral blood mononuclear cell, Ankylosing spondylitis, Disease activity

何亚亚, 陈闪闪, 李永胜, 刘娟. AS患者sLAIR-1和LAIR-1 mRNA的表达及其与疾病活动度的关系[J]. 检验医学, 2022, 37(10): 915-920.

HE Yaya, CHEN Shanshan, LI Yongsheng, LIU Juan. Expressions of sLAIR-1 and LAIR-1 mRNA in AS patients and their relationship with disease activity[J]. Laboratory Medicine, 2022, 37(10): 915-920.

图/表 5

参考文献 18

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基因	上游引物（5'~3'）	下游引物（5'~3'）	产物大小/bp
β-actin	TCCCATCACCATCTTCCAGG	GATGACCCTTTTGGCTCCC	145
LAIR-1	CGCCAGAATCAGATAAAGCAGG	CTGAGCATACGTCACCTCCT	198

基因	上游引物（5'~3'）	下游引物（5'~3'）	产物大小/bp
β-actin	TCCCATCACCATCTTCCAGG	GATGACCCTTTTGGCTCCC	145
LAIR-1	CGCCAGAATCAGATAAAGCAGG	CTGAGCATACGTCACCTCCT	198

组别	例数	吸烟史/%	BMI/（kg/m²）	WBC计数/（×10⁹/L）	LYMPH#/（×10⁹/L）
AS组	81	18（22.22）	23.25±2.95	8.13±1.98	2.72±0.85
正常对照组	80	17（21.25）	22.68±3.24	7.08±1.58	2.28±0.67
统计值		0.149	0.513	4.401	3.528
P值		0.803	0.681	0.005	0.013

组别	例数	吸烟史/%	BMI/（kg/m²）	WBC计数/（×10⁹/L）	LYMPH#/（×10⁹/L）
AS组	81	18（22.22）	23.25±2.95	8.13±1.98	2.72±0.85
正常对照组	80	17（21.25）	22.68±3.24	7.08±1.58	2.28±0.67
统计值		0.149	0.513	4.401	3.528
P值		0.803	0.681	0.005	0.013

组别	例数	sLAIR-1/（ng/mL）	LAIR-1 mRNA	IL-17/（pg/mL）	IL-6/（pg/mL）
AS组	81	5.17±2.37	0.54±0.46	11.37±4.15	22.17±9.13
正常对照组	80	1.72±1.17	1.79±1.12	4.99±1.92	8.08±3.59
统计值		11.680	9.290	12.440	13.010
P值		<0.001	<0.001	<0.001	<0.001