肥胖/超重对哮喘发作期患儿Th1/Th2和Th17/Treg细胞因子的影响

doi:10.3969/j.issn.1673-8640.2023.08.008

摘要/Abstract

摘要：

目的探讨肥胖/超重对哮喘发作期儿童外周血辅助性T细胞（Th）1炎症因子[肿瘤坏死因子α（TNF-α）]、Th2炎症因子[白细胞介素（IL）-10、IL-13]、Th17炎症因子（IL-17F）和调节性T细胞（Treg）炎症因子（IL-35）水平的影响。方法选取2020年1月—2021年12月上海市浦东新区人民医院哮喘患儿112例，根据体重指数（BMI）分为哮喘组（56例）、肥胖/超重合并哮喘组（56例），以肥胖/超重的非哮喘患儿56例（肥胖/超重组）作为对照。检测所有研究对象TNF-α、IL-10、IL-13、IL-17F、IL-35和IL-6、瘦素水平。采用Spearman相关分析评估BMI与各项指标之间的相关性。结果哮喘组、肥胖/超重合并哮喘组和肥胖/超重组之间血清IL-6、IL-10、IL-13和瘦素水平差异均有统计学意义（P<0.05）。肥胖/超重合并哮喘组血清TNF-α水平显著高于哮喘组（P<0.05）。肥胖/超重组血清IL-17F水平显著高于哮喘组和肥胖/超重合并哮喘组（P<0.05）。哮喘组血清IL-35水平显著高于肥胖/超重合并哮喘组和肥胖/超重组（P<0.05）。将肥胖/超重合并哮喘患儿和肥胖/超重非哮喘患儿进一步分为超重合并哮喘组、肥胖合并哮喘组、超重组和肥胖组。Spearman相关分析结果显示，超重合并哮喘组、肥胖合并哮喘组、超重组和肥胖组BMI与IL-6呈正相关（r值分别为0.515、0.510、0.515、0.432，P<0.05）；超重合并哮喘组、肥胖合并哮喘组和肥胖组BMI与瘦素呈正相关（r值分别为0.488、0.578、0.546，P<0.05），与IL-35呈负相关（r值分别为-0.491、-0.517、-0.524，P<0.01）。结论肥胖/超重会引起Th1/Th2表达失衡，肥胖/超重合并哮喘患儿Th1炎症因子呈高表达。

关键词: 肿瘤坏死因子α, 白细胞介素10, 白细胞介素13, 白细胞介素17F, 白细胞介素35, 白细胞介素6, 瘦素, 辅助性T细胞, 肥胖, 哮喘

Abstract:

Objective To investigate the influence of obesity/overweight on T helper（Th） 1 cell inflammatory factor [tumor necrosis factor-alpha（TNF-α）]，Th2 cell inflammatory factors [interleukin（IL）-10，IL-13]，Th17 cell inflammatory factor（IL-17F） and regulatory T cell（Treg） inflammatory factor（IL-35） at attack stage of asthma children in peripheral blood. Methods A total of 112 children with asthma at Shanghai Pudong New Area People's Hospital from January 2020 to December 2021 were enrolled and classified into asthma group（56 cases），obesity/overweight combined asthma group（56 cases） according to body mass index（BMI），and 56 non-asthma children with obesity/overweight（obesity/overweight group） were enrolled. The levels of TNF-α，IL-10，IL-13，IL-17F，IL-35，IL-6 and leptin in peripheral blood were determined. The correlation between BMI and indexes was evaluated by Spearman correlation. Results There was statistical significance in serum levels of IL-6，IL-10，IL-13 and leptin in asthma group，obesity/overweight combined asthma group and obesity/overweight group（P<0.05）. The serum TNF-α level in obesity/overweight combined asthma group was higher than that in asthma group（P<0.05）. The serum IL-17F level in obesity/overweight group was higher than those in asthma group and obesity/overweight combined asthma group（P<0.05）. IL-35 in asthma group was higher than those in obesity/overweight combined asthma group and obesity/overweight group（P<0.05）. The obesity/overweight combined asthma group and obesity/overweight group were further classified into overweight combined asthma group，obesity combined asthma group，overweight group and obesity group. BMI was positively correlated with IL-6 in overweight combined asthma group，obesity combined asthma group，overweight group and obesity group by Spearman correlation（r=0.515，0.510，0.515 and 0.432，P<0.05）. BMI was positively correlated with leptin（r=0.488，0.578 and 0.546，P<0.05） and negatively correlated with IL-35（r=-0.491，-0.517 and -0.524，P<0.01）. Conclusions Obesity/overweight can cause the imbalance of Th1/Th2 expression. Th1 cell inflammatory factors are mainly expressed in children with obesity/ overweight combined asthma.

Key words: Tumor necrosis factor-alpha, Interleukin-10, Interleukin-13, Interleukin-17F, Interleukin-35, Interleukin-6, Leptin, T helper cell, Obesity, Asthma

中图分类号:

R446.1

丁肖媛, 戴劲盛, 焦荣红, 马素丽, 朱海峰, 吴梦雅, 车艳然, 张蕾. 肥胖/超重对哮喘发作期患儿Th1/Th2和Th17/Treg细胞因子的影响[J]. 检验医学, 2023, 38(8): 748-752.

DING Xiaoyuan, DAI Jinsheng, JIAO Ronghong, MA Suli, ZHU Haifeng, WU Mengya, CHE Yanran, ZHANG Lei. Influence of obesity/overweight on inflammatory factors of Th1/Th2 and Th17/Treg in children with asthma attacks[J]. Laboratory Medicine, 2023, 38(8): 748-752.

图/表 2

参考文献 32

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组别	IL-6		IL-35		瘦素		TNF-α		IL-10		IL-13		IL-17F
组别	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值
超重合并哮喘组	0.515	0.017	-0.491	0.024	0.488	0.025	-0.360	0.109	0.082	0.722	-0.082	0.722	-0.158	0.495
肥胖合并哮喘组	0.510	0.002	-0.517	0.001	0.578	0.000	-0.185	0.287	-0.021	0.906	0.360	0.034	0.056	0.748
超重组	0.515	0.020	-0.430	0.059	0.321	0.167	0.009	0.970	-0.116	0.626	-0.330	0.155	-0.106	0.658
肥胖组	0.432	0.009	-0.524	0.001	0.546	0.001	-0.110	0.524	0.223	0.191	-0.150	0.381	-0.075	0.662

组别	IL-6		IL-35		瘦素		TNF-α		IL-10		IL-13		IL-17F
组别	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值
超重合并哮喘组	0.515	0.017	-0.491	0.024	0.488	0.025	-0.360	0.109	0.082	0.722	-0.082	0.722	-0.158	0.495
肥胖合并哮喘组	0.510	0.002	-0.517	0.001	0.578	0.000	-0.185	0.287	-0.021	0.906	0.360	0.034	0.056	0.748
超重组	0.515	0.020	-0.430	0.059	0.321	0.167	0.009	0.970	-0.116	0.626	-0.330	0.155	-0.106	0.658
肥胖组	0.432	0.009	-0.524	0.001	0.546	0.001	-0.110	0.524	0.223	0.191	-0.150	0.381	-0.075	0.662