非炎性中枢神经系统疾病患者脑脊液IL-6参考区间的建立

doi:10.3969/j.issn.1673-8640.2021.06.011

检验医学 ›› 2021, Vol. 36 ›› Issue (6): 627-630.DOI: 10.3969/j.issn.1673-8640.2021.06.011

非炎性中枢神经系统疾病患者脑脊液IL-6参考区间的建立

王子鹏¹, 欧阳长理², 耿德勤³, 王敦敬³()

1.徐州医科大学研究生院,江苏徐州 221000
2.徐州医科大学附属医院核医学科,江苏徐州 221000
3.徐州医科大学附属医院神经内科,江苏徐州 221000

收稿日期:2020-02-19 出版日期:2021-06-30 发布日期:2021-06-30
通讯作者: 王敦敬
作者简介:王敦敬,E-mail：wangdunjing@163.com。
王子鹏,男,1994年生,硕士,医师,主要从事神经内科相关疾病的诊疗工作。

Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases

WANG Zipeng¹, OUYANG Changli², GENG Deqin³, WANG Dunjing³()

1. Graduate School,Xuzhou Medical University,Xuzhou 221000,Jiangsu,China
2. Department of Nuclear Medicine,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,Jiangsu,China
3. Department of Neurology,the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221000,Jiangsu,China

Received:2020-02-19 Online:2021-06-30 Published:2021-06-30
Contact: WANG Dunjing

摘要/Abstract

摘要：

目的建立非炎性中枢神经系统疾病患者脑脊液白细胞介素6（IL-6）的参考区间。方法选取行腰椎穿刺的非炎性中枢神经系统疾病患者159例建立脑脊液IL-6的参考区间。另选取行腰椎穿刺的非炎性中枢神经系统疾病患者23例和中枢神经系统感染患者29例,用于参考区间的验证。结果根据Dixon法计算结果,剔除1个离群值,有效数据为158个。158例非炎性中枢神经系统疾病患者的脑脊液IL-6数据呈正态分布（Z=0.06,P=0.20）,结果为（4.43±1.18）pg/mL。不同性别和年龄之间差异均无统计学意义（P>0.05）,合并各组后采用x+1.96s方式确定参考区间上限为6.74 pg/mL,参考区间为≤6.74 pg/mL。23例非炎性中枢神经系统疾病患者的脑脊液IL-6检测结果为1.50~6.26 pg/mL,所有结果均在建立的参考区间内;29例中枢神经系统感染患者的脑脊液IL-6检测结果为8.43~3 406.00 pg/mL,均高于参考区间上限;参考区间验证通过。结论初步建立了非炎性中枢神经系统疾病患者脑脊液IL-6参考区间,经验证可用于临床。

关键词: 白细胞介素6, 非炎性中枢神经系统疾病, 脑脊液, 参考区间

Abstract:

Objective To establish the reference interval for cerebrospinal fluid interleukin-6（IL-6） in non-inflammatory central nervous system diseases. Methods A total of 159 patients who were diagnosed as non-inflammatory central nervous system diseases by lumbar puncture examination were enrolled. Totally,23 patients who underwent lumbar puncture and were diagnosed as non-inflammatory central nervous system diseases and 29 patients who were diagnosed as central nervous system infection were enrolled for the verification of reference interval. Results According to the calculation result of Dixon method,1 outlier was eliminated,and there were 158 valid data. The cerebrospinal fluid IL-6 results of 158 patients with non-inflammatory central nervous system diseases showed a normal distribution（Z=0.06,P=0.20）,and the result was（4.43±1.18） pg/mL. There was no statistical significance in cerebrospinal fluid IL-6 levels between males and females（P>0.05）. After merging the groups,the upper limit of the reference interval was 6.74 pg/mL,and the reference interval was ≤6.74 pg/mL using the method of x+1.96s. The cerebrospinal fluid IL-6 results of 23 patients with non-inflammatory central nervous system diseases were 1.50-6.26 pg/mL,and all the results were within the established reference interval. The results of cerebrospinal fluid IL-6 in 29 patients with central nervous system infection were 8.43-3 406.00 pg/mL,which were higher than the established reference interval. The reference interval was validated. Conclusions The IL-6 reference interval of cerebrospinal fluid for patients with non-inflammatory central nervous system diseases has been initially established,and it can be used in clinical practice.

Key words: Interleukin-6, Non-inflammatory central nervous system disease, Cerebrospinal fluid, Reference interval

中图分类号:

R446.1

王子鹏, 欧阳长理, 耿德勤, 王敦敬. 非炎性中枢神经系统疾病患者脑脊液IL-6参考区间的建立[J]. 检验医学, 2021, 36(6): 627-630.

WANG Zipeng, OUYANG Changli, GENG Deqin, WANG Dunjing. Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases[J]. Laboratory Medicine, 2021, 36(6): 627-630.

图/表 3

参考文献 14

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组别	例数	IL-6
≤18岁组	22	4.06±1.08
19~29岁组	28	4.20±1.13
30~39岁组	31	4.65±1.17
40~49岁组	31	4.39±0.98
50~59岁组	26	4.38±1.32
>59岁组	20	4.94±1.37
F值		3.142
P值		0.151

组别	例数	IL-6
≤18岁组	22	4.06±1.08
19~29岁组	28	4.20±1.13
30~39岁组	31	4.65±1.17
40~49岁组	31	4.39±0.98
50~59岁组	26	4.38±1.32
>59岁组	20	4.94±1.37
F值		3.142
P值		0.151

疾病	例数	IL-6
非炎性中枢神经系统疾病	23
血管性头痛	12	4.59、3.86、4.26、2.18、4.64、4.54、4.49、1.50、6.26、4.51、4.78、4.03
躯体形式障碍	10	4.46、4.58、4.60、3.87、4.14、3.35、4.94、5.05、3.49、3.65
腰椎间盘突出	1	4.59
中枢神经系统感染	29
结核性脑膜炎	23	8.57、9.37、16.86、11.05、17.13、16.63、17.38、365.90、226.70、8.79、136.00、8.43、27.17、8.43、11.05、13.65、130.90、121.70、16.86、226.70、9.37、7.96、67.70
结核性脑膜炎	4	3406.00、742.70、208.90、90.85
化脓性脑膜炎	2	232.60、173.3

疾病	例数	IL-6
非炎性中枢神经系统疾病	23
血管性头痛	12	4.59、3.86、4.26、2.18、4.64、4.54、4.49、1.50、6.26、4.51、4.78、4.03
躯体形式障碍	10	4.46、4.58、4.60、3.87、4.14、3.35、4.94、5.05、3.49、3.65
腰椎间盘突出	1	4.59
中枢神经系统感染	29
结核性脑膜炎	23	8.57、9.37、16.86、11.05、17.13、16.63、17.38、365.90、226.70、8.79、136.00、8.43、27.17、8.43、11.05、13.65、130.90、121.70、16.86、226.70、9.37、7.96、67.70
结核性脑膜炎	4	3406.00、742.70、208.90、90.85
化脓性脑膜炎	2	232.60、173.3

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非炎性中枢神经系统疾病患者脑脊液IL-6参考区间的建立

Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases

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