IL-1β、IL-6、TNF-α在慢性HBV感染不同临床病程中的临床意义

doi:10.3969/j.issn.1673-8640.2021.08.002

摘要/Abstract

摘要：

目的探讨白细胞介素（IL）-1β、IL-6及肿瘤坏死因子-α（TNF-α）在不同临床病程慢性乙型肝炎病毒（HBV）感染患者中的临床价值。方法选取240例慢性HBV感染患者,根据临床病程分为乙型肝炎病毒携带者（ASC）60例（ASC组）、慢性乙型肝炎（CHB）患者60例（CHB组）、乙型肝炎肝硬化（LC）患者60例（LC组）及原发性肝癌（HCC）患者60例（HCC组）。以60名体检健康者作为正常对照组。检测所有对象血清IL-1β、IL-6、TNF-α、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（TB）、白蛋白（Alb）水平及HBV DNA载量。结果 ASC组、CHB组、LC组和HCC组IL-1β、IL-6及TNF-α水平均显著高于正常对照组（P<0.01）。HCC组IL-1β、IL-6水平显著低于ASC组、CHB组、LC组（P<0.01）,ASC组TNF-α水平显著低于LC组（P<0.01）,其他指标各组间差异均无统计学意义（P>0.05）。ASC组、CHB组IL-1β、IL-6、TNF-α与HBV DNA载量、ALT、AST、TB、Alb均无相关性（P>0.05）。 LC组除IL-6与ALT呈负相关（r=-0.314,P<0.05）外,其他各项指标之间均无相关性（P>0.05）。HCC组除TNF-α与HBV DNA载量呈负相关（r=-0.348,P<0.05）外,其他各项指标之间均无相关性（P>0.05）。结论血清IL-1β、IL-6和TNF-α水平或可用于评估慢性HBV感染患者病情的严重程度。

关键词: 白细胞介素1β, 白细胞介素6, 肿瘤坏死因子-α, 乙型肝炎病毒, 慢性乙型肝炎

Abstract:

Objective To investigate the clinical values of interleukin（IL）-1β,IL-6 and tumor necrosis factor-alpha（TNF-α） in patients with different clinical courses of chronic hepatitis B virus（HBV） infection. Methods Totally,240 patients with chronic HBV infection were enrolled and classified into 60 patients with asymptomatic carriers（ASC）（ASC group）,60 patients with chronic hepatitis B（CHB）（CHB group）,60 patients with liver cirrhosis（LC）（LC group） and 60 patients with hepatocellular carcinoma（HCC）（HCC group）. Sixty healthy subjects were enrolled as healthy control group. Serum IL-1β,IL-6,TNF-α,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,total bilirubin（TB）,albumin（Alb） and HBV DNA load were determined. Results The levels of IL-1β,IL-6 and TNF-α in ASC group,CHB group,LC group and HCC group were higher than those in healthy control group（P<0.01）. The levels of IL-1β and IL-6 in HCC group were lower than those in ASC group,CHB group and LC group（P<0.01）,and the TNF-α levels in ASC group were lower than those in LC group（P<0.01）. There was no statistical significance for the other indicators among the groups（P>0.05）. There was no correlation between IL-1β,IL-6,TNF-α and HBV DNA load,ALT,AST,TB and Alb in ASC group and CHB group（P>0.05）. Except for the negative correlation between IL-6 and ALT in LC group（r=-0.314,P<0.05）,there was no correlation among the other indicators（P>0.05）. Except for the negative correlation between TNF-α and HBV DNA load in HCC group（r=-0.348,P<0.05）,there was no correlation among the other indicators（P>0.05）. Conclusions Serum IL-1β,IL-6 and TNF-α levels may be used to assess the severity of patients with chronic HBV infection.

Key words: Interleukin-1 beta, Interleukin-6, Tumor necrosis factor-alpha, Hepatitis B virus, Chronic hepatitis B

中图分类号:

R446.1

李彩东, 张旭强, 雷志萍, 陈俏丽, 陈璐, 田鹏飞, 段正军. IL-1β、IL-6、TNF-α在慢性HBV感染不同临床病程中的临床意义[J]. 检验医学, 2021, 36(8): 790-794.

LI Caidong, ZHANG Xuqiang, LEI Zhiping, CHEN Qiaoli, CHEN Lu, TIAN Pengfei, DUAN Zhengjun. Clinical values of IL-1β,IL-6 and TNF-α in different clinical courses of chronic HBV infection[J]. Laboratory Medicine, 2021, 36(8): 790-794.

图/表 7

参考文献 19

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组别	例数	IL-1β/（pg/mL）	IL-6/（ng/L）	TNF-α/（ng/L）
ASC组	60	804.80±154.24^*	95.12±12.37^*	64.38±24.89^*▲
CHB组	60	710.43±152.89^*	80.93±10.87^*	82.44±26.54^*
LC组	60	733.26±106.85^*	76.26±10.57^*	108.50±33.64^*
HCC组	60	391.50±38.57^*#	52.14±3.06^*#△▲	85.69±36.26^*
正常对照组	60	118.55±74.05	25.79±5.41	33.57±16.58

组别	例数	IL-1β/（pg/mL）	IL-6/（ng/L）	TNF-α/（ng/L）
ASC组	60	804.80±154.24^*	95.12±12.37^*	64.38±24.89^*▲
CHB组	60	710.43±152.89^*	80.93±10.87^*	82.44±26.54^*
LC组	60	733.26±106.85^*	76.26±10.57^*	108.50±33.64^*
HCC组	60	391.50±38.57^*#	52.14±3.06^*#△▲	85.69±36.26^*
正常对照组	60	118.55±74.05	25.79±5.41	33.57±16.58

项目	ALT		AST		TB		Alb		HBV DNA载量
项目	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值
IL-1β	0.016	0.906	0.193	0.141	-0.113	0.388	0.062	0.39	-0.153	0.281
IL-6	0.103	0.435	0.003	0.984	-0.109	0.408	-0.180	0.170	-0.056	0.736
TNF-α	-0.035	0.789	0.178	0.173	-0.185	0.158	0.051	0.701	0.117	0.357

项目	ALT		AST		TB		Alb		HBV DNA载量
项目	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值
IL-1β	0.016	0.906	0.193	0.141	-0.113	0.388	0.062	0.39	-0.153	0.281
IL-6	0.103	0.435	0.003	0.984	-0.109	0.408	-0.180	0.170	-0.056	0.736
TNF-α	-0.035	0.789	0.178	0.173	-0.185	0.158	0.051	0.701	0.117	0.357

项目	ALT		AST		TB		Alb		HBV DNA载量
项目	r值	P值	r值	P值	r值	P值	r值	P值	r值	P值
IL-1β	0.073	0.581	0.101	0.441	-0.047	0.723	0.102	0.440	0.045	0.733
IL-6	0.062	0.639	0.009	0.946	-0.117	0.372	0.010	0.939	0.124	0.344
TNF-α	-0.111	0.398	-0.169	0.198	-0.028	0.835	-0.021	0.874	-0.164	0.212