肥胖人群外周血嗜酸性粒细胞数量和活性表达

doi:10.3969/j.issn.1673-8640.2023.09.008

摘要/Abstract

摘要：

目的分析肥胖人群外周血嗜酸性粒细胞数量和活性表达情况。方法选取2021年6—9月中国中医科学院广安门医院>40岁的体检者41名，根据体重指数（BMI）分为肥胖组（20例，BMI≥30 kg/m²）和对照组（21例，18.5 kg/m²<BMI<28 kg/m²）。收集所有研究对象一般资料。采用流式细胞术检测外周血嗜酸性粒细胞CD62L、CD63表达情况；采用酶联免疫吸附试验（ELISA）检测血清嗜酸性粒细胞阳离子蛋白（ECP）水平。比较2个组外周血嗜酸性粒细胞数量、百分比和CD62L、CD63、ECP表达的差异。结果肥胖组外周血嗜酸性粒细胞、中性粒细胞数量和百分比、血清ECP水平均稍高于对照组，但差异无统计学意义（P>0.05）；CD62L水平低于对照组（P<0.05），CD63高于对照组（P<0.01）。CD62L与BMI呈显著负相关（r=-0.332，P=0.034）。结论肥胖可能与外周血嗜酸性粒细胞活性显著增强有关。

关键词: 嗜酸性粒细胞, 嗜酸性粒细胞阳离子蛋白, 肥胖

Abstract:

Objective To analyze the number and activity of peripheral blood eosinophils in obese people. Methods A total of 41 subjects >40 years old at Guang' anmen Hospital from June to September 2021 were enrolled. According to body mass index（BMI），the subjects were classified into obese group（20 cases，BMI≥30 kg/m²） and control group（21 cases，18.5 kg/m²<BMI<28 kg/m²）. The general information were collected. The expressions of CD62L and CD63 in peripheral blood eosinophils were determined by flow cytometry. The level of serum eosinophil cationic protein（ECP） was determined by enzyme-linked immunosorbent assay（ELISA）. The number and percentage of peripheral blood eosinophils and the expressions of CD62L，CD63 and ECP were compared between the 2 groups. Results The number and percentage of peripheral blood eosinophils and neutrophils and serum ECP levels in obese group were higher than those in control group（P>0.05）. CD62L was lower than that in control group（P<0.05），and CD63 was higher than that in control group（P<0.01）. CD62L was negatively correlated with BMI（r=-0.332，P=0.034）. Conclusions Obesity might be related to the increase of peripheral blood eosinophil activity.

Key words: Eosinophil, Eosinophil cationic protein, Obesity

中图分类号:

R446.11

程序, 杨忖卿, 庞博, 谷春, 侯雪筠, 费佳欣, 吴敏, 李军, 刘贵建. 肥胖人群外周血嗜酸性粒细胞数量和活性表达[J]. 检验医学, 2023, 38(9): 855-859.

CHENG Xu, YANG Cunqing, PANG Bo, GU Chun, HOU Xueyun, FEI Jiaxin, WU Min, LI Jun, LIU Guijian. Number and activity of peripheral blood eosinophils in obese people[J]. Laboratory Medicine, 2023, 38(9): 855-859.

图/表 3

参考文献 28

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组别	例数	性别		年龄/岁	丙氨酸氨基转移酶/（U/L）	天门冬氨酸氨基转移酶/（U/L）	高密度脂蛋白胆固醇/（mmol/L）	低密度脂蛋白胆固醇/（mmol/L）
组别	例数	男/ [例（%）]	女/ [例（%）]	年龄/岁	丙氨酸氨基转移酶/（U/L）	天门冬氨酸氨基转移酶/（U/L）	高密度脂蛋白胆固醇/（mmol/L）	低密度脂蛋白胆固醇/（mmol/L）
对照组	21	6（28.6）	15（71.4）	62±6	21.00（13.50，29.00）	21.00（17.50，27.00）	1.30±0.23	3.57±0.79
肥胖组	20	5（25.0）	15（75.0）	64±7	19.50（17.00，29.23）	22.00（18.25，24.83）	1.26±0.23	3.22±0.68
统计值		0.067		0.785	0.052	0.091	0.391	0.887
P值		0.538		0.404	0.958	0.927	0.572	0.142
组别	三酰甘油/ （mmol/L）		血糖/（mmol/L）		中性粒细胞计数/（×10⁹/L）	中性粒细胞百分比/%	嗜酸性粒细胞计数/（×10⁹/L）	嗜酸性粒细胞百分比/%
对照组	1.62（1.15，2.25）		5.33（5.00，6.00）		3.63±0.97	56.30（53.65，61.90）	0.11（0.07，0.17）	1.80（1.40，2.90）
肥胖组	1.85（1.55，2.35）		5.60（5.10，6.88）		3.94±1.24	56.05（52.3，69.00）	0.14（0.08，0.28）	2.25（1.40，3.78）
统计值	1.356		0.863		0.901	0.730	1.032	0.953
P值	0.175		0.388		0.376	0.465	0.302	0.341

组别	例数	性别		年龄/岁	丙氨酸氨基转移酶/（U/L）	天门冬氨酸氨基转移酶/（U/L）	高密度脂蛋白胆固醇/（mmol/L）	低密度脂蛋白胆固醇/（mmol/L）
组别	例数	男/ [例（%）]	女/ [例（%）]	年龄/岁	丙氨酸氨基转移酶/（U/L）	天门冬氨酸氨基转移酶/（U/L）	高密度脂蛋白胆固醇/（mmol/L）	低密度脂蛋白胆固醇/（mmol/L）
对照组	21	6（28.6）	15（71.4）	62±6	21.00（13.50，29.00）	21.00（17.50，27.00）	1.30±0.23	3.57±0.79
肥胖组	20	5（25.0）	15（75.0）	64±7	19.50（17.00，29.23）	22.00（18.25，24.83）	1.26±0.23	3.22±0.68
统计值		0.067		0.785	0.052	0.091	0.391	0.887
P值		0.538		0.404	0.958	0.927	0.572	0.142
组别	三酰甘油/ （mmol/L）		血糖/（mmol/L）		中性粒细胞计数/（×10⁹/L）	中性粒细胞百分比/%	嗜酸性粒细胞计数/（×10⁹/L）	嗜酸性粒细胞百分比/%
对照组	1.62（1.15，2.25）		5.33（5.00，6.00）		3.63±0.97	56.30（53.65，61.90）	0.11（0.07，0.17）	1.80（1.40，2.90）
肥胖组	1.85（1.55，2.35）		5.60（5.10，6.88）		3.94±1.24	56.05（52.3，69.00）	0.14（0.08，0.28）	2.25（1.40，3.78）
统计值	1.356		0.863		0.901	0.730	1.032	0.953
P值	0.175		0.388		0.376	0.465	0.302	0.341