Correlations of serum IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP levels with neovascularization glaucoma

doi:10.3969/j.issn.1673-8640.2020.05.008

Abstract

Abstract:

Objective To investigate the correlations of serum interleukin（IL）-1,IL-2,IL-6,IL-8,IL-10 and high-sensitivity C-reactive protein（hs-CRP） levels with neovascularization glaucoma（NVG）. Methods A total of 69 patients with NVG and 39 healthy subjects were enrolled. The levels of IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP were determined. Ophthalmic examination [intraocular pressure（IOP）,central corneal thickness（CCT）,vertical cup-disc ratio（VCDR）,axial length（AL）,anterior chamber depth（ACD）,visual field mean defect（MD）,visual field mean sensitivity（MS） and so on] and physical examination were performed. There were 32 cases of retinal vein occlusion（RVO） secondary NVG and 37 cases of diabetic retinopathy（DR） secondary NVG. Spearman correlation analysis was used to evaluate the correlation between factors and ophthalmic parameters of NVG patients. Binary Logistic regression analysis was performed to identify the risk factors for NVG. Receiver operating characteristic（ROC） curve was used to analyze the value of each parameter in diagnosing NVG. Results Serum levels of IL-6,IL-8 and hs-CRP in NVG group were higher than those in control group（P<0.01）. There was no statistical significance in IL-1,IL-2 and IL-10 between the 2 groups（P>0.05）. There was no statistical significance in IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP levels between DR secondary NVG group and RVO secondary NVG group（P>0.05）. Spearman correlation analysis showed that serum IL-6 level was positively correlated with MD in NVG patients（r=0.257,P<0.001）,and it was negatively correlated with MS（r=-0.196,P<0.001）. Serum IL-1,IL-2,IL-8,IL-10 and hs-CRP levels were not correlated with ophthalmic parameters（P>0.05）. Binary Logistic regression analysis showed that IL-6 [odds ratio（OR）=4.443,95% confidence interval（CI） 1.207-16.40],IL-8（OR=1.067,95%CI 1.001-1.237） and hs-CRP（OR=3.886,95%CI 1.191-12.683）were independent risk factors of NVG. ROC curve showed that the areas under curves of IL-6,IL-8,hs-CRP and the combined determination（IL-6+IL-8+hs-CRP） were 0.773,0.873,0.760 and 0.902,respectively. The efficiency of the combined determination was better than those of single determinations. Conclusions IL-6,IL-8 and hs-CRP are potential for the diagnosis of NVG,which play roles in the development of NVG.

Key words: Interleukin 6, Interleukin 8, High-sensitivity C-reactive protein, Neovascularization glaucoma

CLC Number:

R446.1

SONG Yunxiao, HUANG Xiaofeng, ZHANG Haichen, YAO Tianyue, YUAN Wenhua, ZHAO Zhiyun. Correlations of serum IL-1,IL-2,IL-6,IL-8,IL-10 and hs-CRP levels with neovascularization glaucoma[J]. Laboratory Medicine, 2020, 35(5): 434-439.

Figures/Tables 7

References 20

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组别	例数	糖尿病史/例（%）	高血压史/例（%）	收缩压/kPa	舒张压/kPa	BMI/（kg/m²）
正常对照组	39	12（30.77）	22（56.41）	17.52±2.24	9.86±2.05	23.63±3.07
NVG组	69	48（69.57）^*	38（55.07）	17.69±2.36	10.17±1.82	24.33±3.22

组别	例数	糖尿病史/例（%）	高血压史/例（%）	收缩压/kPa	舒张压/kPa	BMI/（kg/m²）
正常对照组	39	12（30.77）	22（56.41）	17.52±2.24	9.86±2.05	23.63±3.07
NVG组	69	48（69.57）^*	38（55.07）	17.69±2.36	10.17±1.82	24.33±3.22

组别	例数	IL-1/ （pg/mL）	IL-2/ （pg/mL）	IL-6/ （pg/mL）	IL-8/ （pg/mL）	IL-10/ （pg/mL）	hs-CRP/ （mg/L）
正常对照组	39	5.00±0.02	389.15±120.72	2.22±0.37	8.0（7.0~12.0）	5.05±0.32	0.60（0.33~0.99）
NVG组	69	5.34±1.47	430.95±172.76	3.52±2.61^**	23.0（13.5~167.0）^**	5.16±1.28	1.09（0.68~2.64）^*

组别	例数	IL-1/ （pg/mL）	IL-2/ （pg/mL）	IL-6/ （pg/mL）	IL-8/ （pg/mL）	IL-10/ （pg/mL）	hs-CRP/ （mg/L）
正常对照组	39	5.00±0.02	389.15±120.72	2.22±0.37	8.0（7.0~12.0）	5.05±0.32	0.60（0.33~0.99）
NVG组	69	5.34±1.47	430.95±172.76	3.52±2.61^**	23.0（13.5~167.0）^**	5.16±1.28	1.09（0.68~2.64）^*

组别	例数	IL-1/ （pg/mL）	IL-2/ （pg/mL）	IL-6/ （pg/mL）	IL-8/ （pg/mL）	IL-10/ （pg/mL）	hs-CRP/ （mg/L）
DR继发性NVG组	37	5.22±1.20	402.10±147.54	3.25±2.52	21.5（13.3~144.5）	5.09±0.52	1.05（0.68~2.67）
RVO继发性NVG组	32	5.46±1.73	461.79±194.06	3.81±2.73	33.0（15.5~210.0）	5.34±1.85	1.09（0.68~2.64）