Abstract:

Objective To investigate the influence of dry heat inactive treatment（56 ℃ 30 min） for the determination of inflammatory indicators related with corona virus disease 2019（COVID-19）diagnosis and treatment. Methods Totally,332 clinical residual blood samples which C-reactive protein（CRP）,procalcitonin（PCT） and interleukin 6（IL-6） determinations were carried out were collected. All the samples were inactivated by dry heat inactive treatment at 56℃ for 30 min on the day of collection,and the difference of results was compared. Results There was no statistical significance in CRP determination results before and after inactivation（P=0.167）,and there was statistical significance in IL-6 and PCT determination results before and after inactivation（P=0.002,P<0.001）,of which the PCT determination results of 98（79.03%） samples decreased after inactivation. The determination results of the 3 inflammatory indicators before and after inactivation had good consistency,and PCT,CRP and IL-6 had 96.77%,97.11% and 97.11% scatter points in 95% confidence intervals,respectively. The correlation coefficients （r） were 0.992,0.999 and 0.996,respectively. Conclusions Dry heat inactive treatment at 56℃ for 30 min has no effect on CRP determination results,but has effect on PCT and IL-6 determination results. It should pay attention to the pretreatment process of dry heat inactive treatment,in order to reduce the infectious risk of COVID-19 patients' samples.

Key words: Corona virus disease 2019, Dry heat inactive treatment, Inflammatory indicator, C-reactive protein, Procalcitonin, Interleukin 6