Research progress of rapid determination methods for SARS-CoV-2 antigen

doi:10.3969/j.issn.1673-8640.2023.01.019

Abstract

Abstract:

Severe acute respiratory syndrome coronavirus 2（SARS-CoV-2）infection has led to a global health crisis. Rapid and accurate determination of SARS-CoV-2 is an important prerequisite for determining the scale of infection and further taking medical interventions or government actions. Although nucleic acid test is still the standard method for diagnosing corona virus disease 2019（COVID-19），antigen test methods that are easier to achieve point-of-care testing（POCT）are still indispensable additions under some conditions. This review mainly summarizes the research progress of SARS-CoV-2 antigen test POCT methods，such as colloidal gold，latex microspheres，fluorescent material，quantum dot and enzyme，and briefly introduces various types of sensors based on different methods，in order to provide a reference for the development of SARS-CoV-2 diagnostic reagents.

Key words: Severe acute respiratory syndrome coronavirus 2, Corona virus disease 2019, Point-of-care testing, Antigen test

CLC Number:

R373.1

YUE Caidie, LI Junyan, DING Aijun, XIE Li, ZENG Weikun. Research progress of rapid determination methods for SARS-CoV-2 antigen[J]. Laboratory Medicine, 2023, 38(1): 87-93.

Figures/Tables 1

References 37

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传感器类型	研究者	基本原型	靶标	检测优势
电容式生物传感器	GEORGAS等^[33]	将ACE2蛋白固定在金叉指电极表面上的无抗体生物传感器	S蛋白抗原	检测时间为2 min；可区分临床阳性样本（7份）和阴性样本（16份）；检测限为750 pg/（μL·mm²）；复现性>95%
	QI等^[34]	与微电极阵列芯片结合的定量电容式适配体传感器	N蛋白抗原	检测时间为15 s；线性范围为10^-5~10^-2 ng/mL；无标记、实时、易操作；对目标抗原的选择性高达6 369∶1；临床样本（9份阳性、9份阴性）验证均达到预期结果
光学生物传感器	HUANG等^[35]	病毒S蛋白特异性纳米共振传感器	S蛋白抗原	检测时间为15 min；检测限为370 VP/mL；可在酶标仪和移动设备上显示结果；低成本、高通量
	DAI等^[36]	基于激光外差反馈的新型表面等离子体共振生物传感器	S蛋白抗原	检测时间为1 min；线性范围为0.01~1 000 ng/mL；检测限为0.08 pg/mL
压电生物传感器	FORINOVá等^[37]	由抗污染三元共聚物生物接口改良的压电生物传感器	N蛋白抗原	检测时间为20 min；检测限为1.3×10⁴ PFU/mL；经质谱和RT-qPCR验证，特异性高；抗污染能力强

传感器类型	研究者	基本原型	靶标	检测优势
电容式生物传感器	GEORGAS等^[33]	将ACE2蛋白固定在金叉指电极表面上的无抗体生物传感器	S蛋白抗原	检测时间为2 min；可区分临床阳性样本（7份）和阴性样本（16份）；检测限为750 pg/（μL·mm²）；复现性>95%
	QI等^[34]	与微电极阵列芯片结合的定量电容式适配体传感器	N蛋白抗原	检测时间为15 s；线性范围为10^-5~10^-2 ng/mL；无标记、实时、易操作；对目标抗原的选择性高达6 369∶1；临床样本（9份阳性、9份阴性）验证均达到预期结果
光学生物传感器	HUANG等^[35]	病毒S蛋白特异性纳米共振传感器	S蛋白抗原	检测时间为15 min；检测限为370 VP/mL；可在酶标仪和移动设备上显示结果；低成本、高通量
	DAI等^[36]	基于激光外差反馈的新型表面等离子体共振生物传感器	S蛋白抗原	检测时间为1 min；线性范围为0.01~1 000 ng/mL；检测限为0.08 pg/mL
压电生物传感器	FORINOVá等^[37]	由抗污染三元共聚物生物接口改良的压电生物传感器	N蛋白抗原	检测时间为20 min；检测限为1.3×10⁴ PFU/mL；经质谱和RT-qPCR验证，特异性高；抗污染能力强