Abstract: Objective To establish the reference intervals of serum cardiac marker high sensitivity cardiac troponin T (hs-cTnT) and creatine kinase isoenzyme (CK-MB) mass by age and sex in healthy subjects. Methods A total of 5 172 healthy subjects were enrolled and classified into 19 groups (＜28 d, 29 d-＜2 months, 2-＜3 months, 3-＜4 months, 4-＜5 months, 5-＜7 months, 7-＜10 months, 10 months-＜1 year old, 1-2 years old, 3-4 years old, 5-9 years old, 10-19 years old, 20-29 years old, 30-39 years old, 40-49 years old, 50-59 years old, 60-69 years old, 70-79 years old and ＞80 years old) according to age, and hs-cTnT and CK-MB mass were detected by Roche E411 electrochemiluminescence analyzer. The results were analyzed statistically. Results The hs-cTnT levels in the neonatal serum were high,the medians (males: 61.48 pg/mL and females: 68.27 pg/mL)decreased gradually with age and reached the lowest point in the 10-30 years old, and then gradually increased with age. There were statistical significance between males and females in the 20-28 years old (P<0.05). The CK-MB mass in the neonatal serum was the highest and decreased with age, and the lowest point appeared later than the hs-cTnT. Conclusions The hs-cTnT and CK-MB mass in healthy subjects of different age and sex are different. The levels of foreign healthy subjects provided by Roche are not applied. Laboratories should establish the reference intervals according to different age and sex.

Key words: Cardiac marker, High senstitivity cardiac troponin T, Creatine kinase isoenzyme mass, Reference interval, Healthy subject