Establishment of reference interval for the detection of serum protein electrophoresis by V8 Nexus high speed automated capillary electrophoresis apparatus

doi:10.3969/j.issn.1673-8640.2021.01.002

Abstract

Abstract:

Objective To preliminarily establish a reference interval for serum protein electrophoresis（SPE） by V8 Nexus high speed automated capillary electrophoresis（CE） apparatus. Methods A total of 649 healthy subjects were enrolled in this study and classified into 5 groups according to ages,including 18-29-year-old,30-39-year-old,40-49-year-old,50-59-year-old and ≥60-year-old groups. Serum samples were detected by the CE apparatus. The reference interval of CE for SPE detection was evaluated according to the document method of the Health Industry Standard WS/T 402—2012 of the People's Republic of China. Results All data from males and females obeyed normal distribution or approximately normal distribution（P>0.05）. There were statistical differences in the levels of albumin,α₂ globulin,β₁ globulin and γ globulin between males and females（P<0.05）,while there was no statistical significance in the results of α₁globulin,β₂ globulin or β globulin between males and females（P>0.05）. In males,there were statistical differences in the levels of albumin,α₂ globulin and β₁ globulin between 18-59-year-old groups and ≥60-year-old group（P<0.05）,but there was no statistical significance in these parameters between 18-59-year-old groups（P>0.05）;neither were α₁ globulin,β₂ globulin,γ globulin or β globulin between any groups（P>0.05）. In females,there were statistical differences in the levels of albumin,β₁ globulin,β₂ globulin and β globulin between 18-59-year-old groups and ≥60-year-old group（P<0.05）,but there was no statistical significance in these parameters between 18-59-year-old groups（P>0.05）;neither were α₁ globulin,α₂ globulin or γ globulin between any groups（P>0.05）. According to the results of LSD test,SNK test and Z test,the age groups could be combined into one group to establish the reference interval of SPE. Conclusion The reference interval of SPE has been preliminarily established by V8 Nexus high speed automated CE apparatus. Clinical laboratories should establish a reference interval for different sexes or ages according to actual situation.

Key words: Serum protein electrophoresis, Capillary electrophoresis, Reference interval

CLC Number:

R446.1

AN Chongwen, LI Haixia, JIAO Lili. Establishment of reference interval for the detection of serum protein electrophoresis by V8 Nexus high speed automated capillary electrophoresis apparatus[J]. Laboratory Medicine, 2021, 36(1): 8-12.

Figures/Tables 5

References 12

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性别	例数	白蛋白	α₁球蛋白	α₂球蛋白	β₁球蛋白	β₂球蛋白	γ球蛋白	β球蛋白
男性	318	64.11±2.84	3.72±0.51	5.94±1.30	6.43±0.86	4.29±0.73	15.51±2.39	10.71±1.32
女性	331	61.87±2.75^*	3.80±0.57	6.74±1.23^*	6.66±0.75^*	4.22±0.65	16.72±2.37^*	10.87±1.14
合计	649	62.96±3.01	3.76±0.54	6.35±1.32	6.54±0.81	4.25±0.69	16.13±2.46	10.80±1.23

性别	例数	白蛋白	α₁球蛋白	α₂球蛋白	β₁球蛋白	β₂球蛋白	γ球蛋白	β球蛋白
男性	318	64.11±2.84	3.72±0.51	5.94±1.30	6.43±0.86	4.29±0.73	15.51±2.39	10.71±1.32
女性	331	61.87±2.75^*	3.80±0.57	6.74±1.23^*	6.66±0.75^*	4.22±0.65	16.72±2.37^*	10.87±1.14
合计	649	62.96±3.01	3.76±0.54	6.35±1.32	6.54±0.81	4.25±0.69	16.13±2.46	10.80±1.23

组别	例数	白蛋白	α₁球蛋白	α₂球蛋白	β₁球蛋白	β₂球蛋白	γ球蛋白	β球蛋白
18~29岁组	63	64.54±2.94^*	3.75±0.53	6.27±1.57^*	6.15±0.93^*	4.25±0.75	15.05±2.35	10.41±1.42
30~39岁组	113	64.32±2.55^*	3.67±0.50	5.78±1.22^*	6.36±0.88^*	4.31±0.68	15.57±2.24	10.67±1.29
40~49岁组	74	64.06±3.04^*	3.66±0.47	5.74±1.15^*	6.61±0.81^*	4.20±0.79	15.73±2.44	10.80±1.31
50~59岁组	46	63.91±2.40^*	3.83±0.46	5.99±1.10^*	6.60±0.68^*	4.39±0.76	15.28±2.19	10.99±1.23
≥60岁	22	62.43±3.60	3.85±0.65	6.42±1.51	6.60±0.81	4.37±0.63	16.33±3.27	10.97±1.24

组别	例数	白蛋白	α₁球蛋白	α₂球蛋白	β₁球蛋白	β₂球蛋白	γ球蛋白	β球蛋白
18~29岁组	63	64.54±2.94^*	3.75±0.53	6.27±1.57^*	6.15±0.93^*	4.25±0.75	15.05±2.35	10.41±1.42
30~39岁组	113	64.32±2.55^*	3.67±0.50	5.78±1.22^*	6.36±0.88^*	4.31±0.68	15.57±2.24	10.67±1.29
40~49岁组	74	64.06±3.04^*	3.66±0.47	5.74±1.15^*	6.61±0.81^*	4.20±0.79	15.73±2.44	10.80±1.31
50~59岁组	46	63.91±2.40^*	3.83±0.46	5.99±1.10^*	6.60±0.68^*	4.39±0.76	15.28±2.19	10.99±1.23
≥60岁	22	62.43±3.60	3.85±0.65	6.42±1.51	6.60±0.81	4.37±0.63	16.33±3.27	10.97±1.24

组别	例数	白蛋白	α₁球蛋白	α₂球蛋白	β₁球蛋白	β₂球蛋白	γ球蛋白	β球蛋白
18~29岁组	86	62.35±2.25^*	3.79±0.54	6.83±1.18	6.49±0.78^*	4.15±0.65^*	16.39±2.01	10.65±1.21^*
30~39岁组	105	62.32±2.47^*	3.76±0.58	6.62±1.18	6.58±0.74^*	4.14±0.58^*	16.58±2.30	10.72±1.07^*
40~49岁组	86	61.23±3.02^*	3.85±0.59	6.63±1.21	6.85±0.78^*	4.24±0.64^*	17.20±2.34	11.09±1.10^*
50~59岁组	36	61.34±2.95^*	3.73±0.52	6.88±1.34	6.74±0.58^*	4.36±0.80^*	16.95±3.17	11.10±1.16^*
≥60岁	18	61.03±2.68	3.96±0.56	7.30±1.43	6.76±0.60	4.60±0.67	16.35±2.59	11.36±1.05