Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases

doi:10.3969/j.issn.1673-8640.2021.06.011

Abstract

Abstract:

Objective To establish the reference interval for cerebrospinal fluid interleukin-6（IL-6） in non-inflammatory central nervous system diseases. Methods A total of 159 patients who were diagnosed as non-inflammatory central nervous system diseases by lumbar puncture examination were enrolled. Totally,23 patients who underwent lumbar puncture and were diagnosed as non-inflammatory central nervous system diseases and 29 patients who were diagnosed as central nervous system infection were enrolled for the verification of reference interval. Results According to the calculation result of Dixon method,1 outlier was eliminated,and there were 158 valid data. The cerebrospinal fluid IL-6 results of 158 patients with non-inflammatory central nervous system diseases showed a normal distribution（Z=0.06,P=0.20）,and the result was（4.43±1.18） pg/mL. There was no statistical significance in cerebrospinal fluid IL-6 levels between males and females（P>0.05）. After merging the groups,the upper limit of the reference interval was 6.74 pg/mL,and the reference interval was ≤6.74 pg/mL using the method of x+1.96s. The cerebrospinal fluid IL-6 results of 23 patients with non-inflammatory central nervous system diseases were 1.50-6.26 pg/mL,and all the results were within the established reference interval. The results of cerebrospinal fluid IL-6 in 29 patients with central nervous system infection were 8.43-3 406.00 pg/mL,which were higher than the established reference interval. The reference interval was validated. Conclusions The IL-6 reference interval of cerebrospinal fluid for patients with non-inflammatory central nervous system diseases has been initially established,and it can be used in clinical practice.

Key words: Interleukin-6, Non-inflammatory central nervous system disease, Cerebrospinal fluid, Reference interval

CLC Number:

R446.1

WANG Zipeng, OUYANG Changli, GENG Deqin, WANG Dunjing. Establishment of cerebrospinal fluid IL-6 reference interval in non-inflammatory central nervous system diseases[J]. Laboratory Medicine, 2021, 36(6): 627-630.

Figures/Tables 3

References 14

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组别	例数	IL-6
≤18岁组	22	4.06±1.08
19~29岁组	28	4.20±1.13
30~39岁组	31	4.65±1.17
40~49岁组	31	4.39±0.98
50~59岁组	26	4.38±1.32
>59岁组	20	4.94±1.37
F值		3.142
P值		0.151

组别	例数	IL-6
≤18岁组	22	4.06±1.08
19~29岁组	28	4.20±1.13
30~39岁组	31	4.65±1.17
40~49岁组	31	4.39±0.98
50~59岁组	26	4.38±1.32
>59岁组	20	4.94±1.37
F值		3.142
P值		0.151

疾病	例数	IL-6
非炎性中枢神经系统疾病	23
血管性头痛	12	4.59、3.86、4.26、2.18、4.64、4.54、4.49、1.50、6.26、4.51、4.78、4.03
躯体形式障碍	10	4.46、4.58、4.60、3.87、4.14、3.35、4.94、5.05、3.49、3.65
腰椎间盘突出	1	4.59
中枢神经系统感染	29
结核性脑膜炎	23	8.57、9.37、16.86、11.05、17.13、16.63、17.38、365.90、226.70、8.79、136.00、8.43、27.17、8.43、11.05、13.65、130.90、121.70、16.86、226.70、9.37、7.96、67.70
结核性脑膜炎	4	3406.00、742.70、208.90、90.85
化脓性脑膜炎	2	232.60、173.3

疾病	例数	IL-6
非炎性中枢神经系统疾病	23
血管性头痛	12	4.59、3.86、4.26、2.18、4.64、4.54、4.49、1.50、6.26、4.51、4.78、4.03
躯体形式障碍	10	4.46、4.58、4.60、3.87、4.14、3.35、4.94、5.05、3.49、3.65
腰椎间盘突出	1	4.59
中枢神经系统感染	29
结核性脑膜炎	23	8.57、9.37、16.86、11.05、17.13、16.63、17.38、365.90、226.70、8.79、136.00、8.43、27.17、8.43、11.05、13.65、130.90、121.70、16.86、226.70、9.37、7.96、67.70
结核性脑膜炎	4	3406.00、742.70、208.90、90.85
化脓性脑膜炎	2	232.60、173.3

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