Abstract:

Objective To establish the reference intervals of serum pepsinogenⅠ（PGⅠ）, pepsinogenⅡ（PGⅡ） and the ratio of pepsinogenⅠ/pepsinogenⅡ（PGR） for apparently healthy subjects in Zigong,and to provide a reference for clinical diagnosis and treatment. Methods The levels of PGⅠ and PGⅡ were determined by latex enhanced immunoturbidimetry,¹³C urea breath test was used to screen Helicobacter pylori（HP） infection in 2 880 apparently healthy subjects from Zigong First People's Hospital,and PGR was calculated. The reference interval was established by percentile method（P_2.5-P_97.5）. The efficacy of PGⅠand PG Ⅱin judging HP positivity was evaluated by receiver operating characteristic（ROC） curve. Kendall's tau-b correlation analysis was used to evaluate the correlation between PGⅠ,PGⅡ and HP positivity. Results The levels of serum PGⅠand PGⅡin males were higher than those in females（P<0.05）,and PGR was lower（P<0.05）. The levels of serum PGⅠand PGⅡ in 18-44-year-old group,45-59-year-old group and >59-year-old group increased in turn（P<0.05）. There was no statistical significance for PGR among the age groups（P>0.05）. The reference intervals of serum PGⅠand PGⅡ were established according to sex and age,and the reference intervals of PGR were established according to sex. Taking the positive ¹³C urea breath test as the basis for HP positivity,the areas under curves for HP positivity judged by PGⅠ,PGⅡ and PGR were 0.712,0.825 and 0.799,respectively. There were correlations between PGⅠ,PGⅡ and HP positivity（r=0.286 and 0.440,respectively,P<0.01）,while PGR was negatively correlated with HP positivity（r=-0.403,P<0.01）. Conclusions The reference intervals of serum PGⅠ,PGⅡ and PGR in apparently healthy subjects in Zigong have been established.

Key words: Pepsinogen Ⅰ, Pepsinogen Ⅱ, Reference interval