Establishment of the reference intervals of leukocyte surface CD64 and HLA-DR indexes for healthy adults and related factors

doi:10.3969/j.issn.1673-8640.2022.03.010

Abstract

Abstract:

Objective To establish the reference intervals of leukocyte surface CD64 and HLA-DR indexes for healthy adults,and to investigate the factors on the reference intervals. Methods Totally,126 healthy subjects aged 22 to 60 years,including 69 males and 57 females,were enrolled from Peking University First Hospital. The expressions of CD64 and HLA-DR on the surface of leukocytes were determined by flow cytometry. Some related indicators were calculated through formula. The reference intervals of CD64 and HLA-DR were established. The influence of age and sex on the reference intervals was investigated. Results There was no statistical significance between different ages and CD64（P>0.05）. The mean fluorescence intensity of monocyte HLA-DR in 18-29-year-old group was lower than those in 40-49-year-old and 50-59-year-old groups（P>0.05,P=0.023,P=0.007）. The mean fluorescence intensity of monocyte HLA-DR in 18-29-year-old group was lower than that in 50-59-year-old group（P=0.034）.There was no statistical significance in leukocyte surface CD64 and HLA-DR indexes between males and females（P>0.05）. Conclusions The reference intervals of leukocyte surface CD64 and HLA-DR indexes for healthy adults have been established. Age may influence the results of leukocyte surface HLA-DR in healthy adults.

Key words: CD64, HLA-DR, Reference interval, Flow cytometry

CLC Number:

R446.11

MIAO Linzi, LU Yao, QU Chenxue, YOU Ran, GONG Yan. Establishment of the reference intervals of leukocyte surface CD64 and HLA-DR indexes for healthy adults and related factors[J]. Laboratory Medicine, 2022, 37(3): 240-245.

Figures/Tables 3

References 25

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项目	中位数	最小值	最大值
中性粒细胞CD64平均荧光强度	809.50	312.00	6 045.00
中性粒细胞CD64荧光强度中位数	601.50	221.00	5 702.00
CD64指数平均荧光强度	0.39	0.05	5.58
中性粒细胞与单核细胞CD64比值%平均荧光强度	5.36	2.17	18.27
中性粒细胞与淋巴细胞CD64比值平均荧光强度	7.46	2.22	30.53
CD64百分比平均荧光强度	4.75	1.34	21.63
CD64指数荧光强度中位数	0.27	0.05	12.13
中性粒细胞与单核细胞CD64比值%荧光强度中位数	4.26	1.76	17.66
中性粒细胞与淋巴细胞CD64比值荧光强度中位数	6.59	2.63	68.70
CD64百分比荧光强度中位数	3.71	1.11	21.14
单核细胞HLA-DR平均荧光强度	32 771.00	14 917.00	78 337.00
单核细胞HLA-DR荧光强度中位数	20 024.50	8 303.00	57 028.00
单核细胞与淋巴细胞HLA-DR比值平均荧光强度	2.94	1.24	6.13
单核细胞与中性粒细胞HLA-DR比值平均荧光强度	53.97	24.45	109.44
单核细胞与淋巴细胞HLA-DR比值荧光强度中位数	62.13	11.02	491.88
单核细胞与中性粒细胞HLA-DR比值荧光强度中位数	36.47	14.22	88.37

项目	中位数	最小值	最大值
中性粒细胞CD64平均荧光强度	809.50	312.00	6 045.00
中性粒细胞CD64荧光强度中位数	601.50	221.00	5 702.00
CD64指数平均荧光强度	0.39	0.05	5.58
中性粒细胞与单核细胞CD64比值%平均荧光强度	5.36	2.17	18.27
中性粒细胞与淋巴细胞CD64比值平均荧光强度	7.46	2.22	30.53
CD64百分比平均荧光强度	4.75	1.34	21.63
CD64指数荧光强度中位数	0.27	0.05	12.13
中性粒细胞与单核细胞CD64比值%荧光强度中位数	4.26	1.76	17.66
中性粒细胞与淋巴细胞CD64比值荧光强度中位数	6.59	2.63	68.70
CD64百分比荧光强度中位数	3.71	1.11	21.14
单核细胞HLA-DR平均荧光强度	32 771.00	14 917.00	78 337.00
单核细胞HLA-DR荧光强度中位数	20 024.50	8 303.00	57 028.00
单核细胞与淋巴细胞HLA-DR比值平均荧光强度	2.94	1.24	6.13
单核细胞与中性粒细胞HLA-DR比值平均荧光强度	53.97	24.45	109.44
单核细胞与淋巴细胞HLA-DR比值荧光强度中位数	62.13	11.02	491.88
单核细胞与中性粒细胞HLA-DR比值荧光强度中位数	36.47	14.22	88.37

组别	例数	单核细胞HLA-DR 平均荧光强度	单核细胞HLA-DR平均荧光强度中位数	单核细胞与中性粒细胞HLA-DR比值（平均荧光强度）
22~29岁组	28	28 170.00 （23 584.25~ 34 420.75）	17 283.50 （14 710.25~22 446.00）	50.99（38.02~59.12）
30~39岁组	22	31 570.00 （27 166.00~ 35 340.50）	18 436.50 （16 611.75~23 400.00）	50.53（45.39~55.69）
40~49岁组	28	37 068.00 （26 443.50~ 43 134.00）	23 774.00 （16 183.75~28 375.00）	54.13（49.13~66.07）
50~60岁组	48	34 905.00 （30 266.25~ 40 171.50）	21 223.50 （18 311.00~26 141.25）	58.28（49.34~69.36）

组别	例数	单核细胞HLA-DR 平均荧光强度	单核细胞HLA-DR平均荧光强度中位数	单核细胞与中性粒细胞HLA-DR比值（平均荧光强度）
22~29岁组	28	28 170.00 （23 584.25~ 34 420.75）	17 283.50 （14 710.25~22 446.00）	50.99（38.02~59.12）
30~39岁组	22	31 570.00 （27 166.00~ 35 340.50）	18 436.50 （16 611.75~23 400.00）	50.53（45.39~55.69）
40~49岁组	28	37 068.00 （26 443.50~ 43 134.00）	23 774.00 （16 183.75~28 375.00）	54.13（49.13~66.07）
50~60岁组	48	34 905.00 （30 266.25~ 40 171.50）	21 223.50 （18 311.00~26 141.25）	58.28（49.34~69.36）

项目	例数	参考区间
中性粒细胞CD64平均荧光强度	126	334.00~2 543.20
中性粒细胞CD64荧光强度中位数	126	240.05~2 195.58
CD64指数平均荧光强度	126	0.06~2.73
中性粒细胞与单核细胞CD64比值%平均荧光强度	126	2.44~14.76
中性粒细胞与淋巴细胞CD64比值平均荧光强度	126	2.44~19.29
CD64百分比平均荧光强度	126	1.50~15.98
CD64指数荧光强度中位数	126	0.05~3.01
中性粒细胞与单核细胞CD64比值%荧光强度中位数	126	1.90~12.96
中性粒细胞与淋巴细胞CD64比值荧光强度中位数	126	2.71~23.21
CD64百分比荧光强度中位数	126	1.27~14.25
单核细胞HLA-DR平均荧光强度
18~39岁	50	15 453.80~56 299.53
40~59岁	76	23 477.95~70 702.98
单核细胞HLA-DR荧光强度中位数
18~49岁	78	9 546.13~39 137.40
50~59岁	48	14 691.85~56 209.68
单核细胞与淋巴细胞HLA-DR比值平均荧光强度	126	1.31~5.96
单核细胞与中性粒细胞HLA-DR比值平均荧光强度	126	34.08~99.80
单核细胞与淋巴细胞HLA-DR比值荧光强度中位数	126	13.18~287.35
单核细胞与中性粒细胞HLA-DR比值荧光强度中位数	126	19.99~76.47