Abstract:

Objective To establish the reference intervals of 6 items of peripheral blood reticulocyte（RET） and related parameters of 0-3 d neonates in Wuhan. Methods From June 2018 to December 2018,406 0-3 d neonates in the Obstetrics Ward of Tongji Hospital of Huazhong Univerisity of Science and Technology Tongji Medical College were enrolled. A total of 6 items,including the percentage of reticulocyte（RET%）,the absolute value of reticulocyte（RET#）,high fluorescent ratio（HFR%）,middle fluorescent ratio（MFR%）,low fluorescent ratio（LFR%） and reticulocyte hemoglobin（RET-He）,were determined for establishing the reference intervals. $\bar{x}$±1.96s was used as biological reference interval. Results The 6 items showed statistical significance between different days（P<0.05）. Except for HFR%,the other 5 items showed no statistical significance between sex（P>0.05）. The reference intervals of RET and related parameters of 0-3 d neonates in Wuhan were as follows. RET%：2.63%-6.27% on the 1st day,2.55%-6.35% on the 2nd day,and 2.06%-6.06% on the 3rd day. RET#：（0.139-0.397）×10¹²/L on the 1st day,（0.150-0.400）×10¹²/L on the 2nd day,and （0.124-0.386）×10¹²/L on the 3rd day. HFR%（male）：12.16%-30.97% on the 1st day,7.64%-29.30% on the 2nd day,and 4.85%-20.64% on the 3rd day;HFR%（female）：10.64%-27.67% on the 1st day,6.78%-25.10% on the 2nd day,and 2.06%-19.26% on the 3rd day. MFR%：13.48%-25.73% on the 1st day,13.24%-26.61% on the 2nd day,and 11.45%-23.00% on the 3rd day. LFR%：48.67%-71.07% on the 1st day,48.24%-77.26% on the 2nd day,and 58.38%-83.56% on the 3rd day. RET-He：29.58-37.30 pg on the 1st day,29.25-36.23 pg on the 2nd day,and 27.49-34.85 pg on the 3rd day. Conclusions Due to different regions and ages,there are differences in the reference intervals of RET. The reference intervals of 6 items of RET and related parameters for 0-3 d neonates in Wuhan have established preliminarily,providing a reference for the diagnosis and treatment of anaemic diseases.

Key words: Neonate, Reticulocyte, Reference interval