Abstract:

Objective To investigate the levels of reticulocyte（RET） parameters in peripheral blood of acute pancreatitis（AP） patients and its clinical significance. Methods A total of 86 hospitalized AP patients,including 51 mild cases and 35 severe cases（7 deaths during hospitalization）,and 50 healthy individuals（control group） who underwent physical examination were enrolled in this study. Peripheral venous blood was collected in AP patients on day 1,5 and 10 of admission and in healthy individuals during physical examination. The levels of RET parameters including reticulocyte（RET）,reticulocyte percentage（RET%）,immature reticulocyte fraction（IRF）,low fluorescence reticulocyte（LFR）,medium fluorescence reticulocyte（MFR） and high fluorescence reticulocyte（HFR）were detected. The receiver operating characteristics（ROC） curves were used to analyze the diagnostic value of IRF,LFR and HFR for AP. Results The levels of RET,RET%,IRF,MFR and HFR in AP group on day 1,5 and 10 of admission were higher than those in the control group,while LFR level was on the contrary. In mild and severe AP patients,RET,RET%,IRF and HFR on day 5 and 10 were higher than those on day 1,while LFR level was lower than that on day 1. On the 10th day of admission,except for the increased LFR,the levels of other parameters in mild and severe patients were decreased compared with those on day 5 of admission（P<0.05）. ROC curve analysis showed that the area under curve（AUC）of the combined detection of IRF,LFR and HFR was 0.922 with sensitivity of 86% and specificity of 92%,which were higher than single detection. Conclusion The levels of RET parameters can reflect the severity of AP. Combined detection of IRF,LFR and HFR may be an effective diagnostic marker for early AP.

Key words: Reticulocyte parameters, Acute pancreatitis, Combined diagnosis