The discussion of applying precipitation to eliminate the state of chyle for HLAP patients' specimens

doi:10.3969/j.issn.1673-8640.2014.09.019

Abstract

Abstract: Objective To investigate phosphotungstic acid magnesium precipitation eliminating effects on hyperlipidemic acute pancreatitis(HLAP) patient's specimens in severe state of chyle before biochemical tests. Methods The normal appearance specimens undergoing phosphotungstic acid magnesium treatment were collected to do biochemical tests, and the correlation data before and after tests were analyzed. The 3 different, mild, moderate and severe, groups of chylous sera with intralipid were performed to do recovery test, and the recovery rates of all biochemical projects were calculated. HLAP patients' specimens were collected to do empirical test, and the chyle eliminating effect and biochemical test effect were observed. Results Except for CO₂ combining ability(CO₂CP), total bilirubin(TBil), direct bilirubin(DBil), creatine kinase-MB(CK-MB), lactate dehydrogenase 1(LD-1), cystatin C(Cys C), high-sensitivity C-reactive protein(hs-CRP), K^＋, Na^＋, Cl^－, Ca^2＋, glucose(Glu), urea, uric acid(UA), creatinine(Cr), amylase(AMY), alanine aminotransferase(ALT), aspartate aminotransferase(AST), gamma-glutamyl transpeptidase(GGT), alkaline phosphatase(ALP), cholinesterase(CHE), total protein(TP), albumin(Alb), total bile acid(TBA), creatine kinase(CK), lactate dehydrogenase(LD) and alpha-hydroxybutyrate dehydrogenase(α-HBDH) can be correctly detected after phosphotungstic acid magnesium treatment, and it showed obvious linear correlation before the treatment.Fitted curve showed that the P was <0.01. In the 3 groups of chylous sera, the lowest recovery rate was 98.03%, the highest recovery rate was 103.44%, and it showed a good recovery effect. The correctly detected biochemical projects of HLAP patients' specimens by phosphotungstic acid magnesium were consistent with the correlation tests of the normal appearance specimens. The specimens of triglyceride (TG)＜10 mmol/L or TG＞40 mmol/L had obvious chyle eliminating effects, and the specimens with TG 10-40 mmol/L needed to be diluted to TG<10 mmol/L before processing. Conclusions Phosphotungstic acid magnesium precipitation has an obvious effect on eliminating blood lipid. It won't interfere most of the biochemical tests. The treatment can be applied before the biochemical tests for HLAP patients' specimens in severe state of chyle.

Key words: Precipitation, Phosphotungstic acid magnesium, Hyperlipidemic acute pancreatitis, Biochemical test

CLC Number:

R446.1

ZHANG Huiliang, HUANG Shuhua, LIU Jianling, XIA Houcai. The discussion of applying precipitation to eliminate the state of chyle for HLAP patients' specimens[J]. , 2014, 29(9): 955-960.

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