Abstract:

Objective To investigate the plasmocyte immuno-phenotype characteristics of CD45-positive（CD45⁺） multiple myeloma（MM） and its difference with the plasmocyte immuno-phenotype characteristics of CD45-negative（CD45^-）MM,and to analyze the correlation of CD45 expression with MM clinical characteristic.Methods The data of 192 MM patients were reviewed for immuno-phenotype analysis.Results According to CD45 expression,192 MM patients were classified into CD45⁺ MM（CD45 average fluorescence intensity on plasma cells >10²） group（37 cases） and CD45^- MM group（155 cases）. In CD45⁺ MM group,the positive rates of CD138,CD38,CD19,CD56,CD20,CD71,CD117 and CD33 were 83.8%,97.3%,32.4%,64.9%,21.6%,51.4%,35.1% and 24.3%. The positive rates of κ and λ light chains were 45.9%. There was 8.2% with no light chain expression. The positive rates of CD19,CD71 and CD138 had statistical significance between the 2 groups（P<0.05）. There was no statistical significance between the 2 groups for the positive rates and light chain expression of CD38,CD56,CD20,CD117 and CD33（P>0.05）. Conclusions For CD45⁺ MM patients,their CD19 and CD71 positive rates increase,and CD138 positive rate decreases. CD45⁺ MM cells may be in the early differentiation stage of plasma cells.

Key words: CD45, Immuno-phenotype, Multiple myeloma