Abstract: Objective To report a case of T cell prolymphocytic leukemia (T-PLL) transforming to anaplastic lymphoma kinase (ALK)-negative anaplastic large cell lymphoma (ALCL, ALK^-), and to review the literatures. Methods The MIC criteria was adopted. Morphology included bone marrow smear (wright staining), pleural effusion smear and cell block, lymph node biopsy, cell chemical dyeing. R banding technique was used in cell genetics analysis, and the immuno-phenotype was analyzed by multicolor flow cytometry. Results The patient was onset as T-PLL, and the abnormal phenotypes in the bone marrow were CD2⁺, CD3⁺, cCD3⁺, CD4⁺, CD7⁺, CD8^-, CD10^-, HLA-DR⁺, cTDT^-, TCRα/β⁺ and CD38, CD5 partly positive. Karyotype analysis showed 46, XX. After 6 months, abnormal phenotypes had been changed into ALCL, and karyotype analysis showed 94, XXX, -X, 1q-x2,+3mar. Conclusions T-PLL can transform into ALCL, ALK^- .

Key words: T cell prolymphocytic leukemia, Anaplastic large cell lympoma, Anaplasticlymphoma kinase, Immuno-phenotype, Karyotype