Bone marrow cell morphology and immuno-phenotype characteristics of MPAL by flow cytometry

doi:10.3969/j.issn.1673-8640.2019.03.010

Abstract

Abstract:

Objective To evaluate the bone marrow cell morphology and immuno-phenotype characteristics of mixed phenotype acute leukemia （MPAL）patients by flow cytometry,and to provide a reference for the diagnosis of leukemia. Methods The clinical data of 9 MPAL patients were collected,and their bone marrow cell morphology and immuno-phenotype characteristics were evaluated. Results The CD34 positive expression rate of 9 MPAL patients was 77.78%. The CD34 positive expression rates of acute myeloid and lymphoid monophenotypic leukemias were 51.74% and 60.00%,respectively. The immuno-phenotypes were mainly expressed in cMPO,cCD79a and CD3. Conclusions The characteristics of MPAL are complex. The results of bone marrow cell morphology and immuno-phenotype by flow cytometry should be analyzed combinedly in order to improve the diagnostic accuracy of MPAL.

Key words: Cell morphology, Immuno-phenotype, Mixed phenotype acute leukemia

CLC Number:

R446.1

WANG Xia, ZHANG Bin. Bone marrow cell morphology and immuno-phenotype characteristics of MPAL by flow cytometry[J]. Laboratory Medicine, 2019, 34(3): 235-239.

病例	CD34	CD38	HLA-DR	CD117	CD13	CD33		CD11b		CD15	CD56
1	86.83	5.99	83.56	5.79	34.76	3.01		0.76		4.04	53.70
2	16.15	26.49	30.02	10.03	25.56	18.58		40.16		14.56	12.65
3	93.09	98.49	87.13	80.40	84.69	91.17		14.43		57.25	60.42
4	31.12	95.08	96.62	44.62	93.87	96.49		36.69		97.28	3.64
5	92.76	91.32	97.87	5.46	11.18	11.49		0.01		17.24	1.06
6	17.82	99.65	99.63	21.98	39.92	0.21		1.04		6.02	3.86
7	40.38	98.67	83.74	84.66	96.88	57.94		13.54		18.56	5.38
病例	CD64	cMPO	CD7	CD9	CD10	CD19	CD22		CD25	cCD79a	TdT
1	1.22	92.37	1.38	89.17	72.44	86.32	9.40		5.76	22.91	44.80
2	32.00	48.56	8.78	17.15	4.31	32.47	16.18		33.74	17.41	7.03
3	32.00	11.04	31.24	7.98	1.58	4.25	32.91		43.87	15.20	70.22
4	81.97	92.47	3.14	1.62	61.16	29.27	6.75		2.51	72.59	11.25
5	4.20	90.54	59.76	6.21	90.45	81.89	15.24		6.58	78.63	5.04
6	20.57	66.13	2.00	99.44	49.79	99.33	99.20		21.68	97.83	29.40
7	15.84	64.03	0.54	10.33	1.28	77.23	8.38		2.08	45.56	1.68

病例	CD34	CD38	HLA-DR	CD117	CD13	CD33		CD11b		CD15	CD56
1	86.83	5.99	83.56	5.79	34.76	3.01		0.76		4.04	53.70
2	16.15	26.49	30.02	10.03	25.56	18.58		40.16		14.56	12.65
3	93.09	98.49	87.13	80.40	84.69	91.17		14.43		57.25	60.42
4	31.12	95.08	96.62	44.62	93.87	96.49		36.69		97.28	3.64
5	92.76	91.32	97.87	5.46	11.18	11.49		0.01		17.24	1.06
6	17.82	99.65	99.63	21.98	39.92	0.21		1.04		6.02	3.86
7	40.38	98.67	83.74	84.66	96.88	57.94		13.54		18.56	5.38
病例	CD64	cMPO	CD7	CD9	CD10	CD19	CD22		CD25	cCD79a	TdT
1	1.22	92.37	1.38	89.17	72.44	86.32	9.40		5.76	22.91	44.80
2	32.00	48.56	8.78	17.15	4.31	32.47	16.18		33.74	17.41	7.03
3	32.00	11.04	31.24	7.98	1.58	4.25	32.91		43.87	15.20	70.22
4	81.97	92.47	3.14	1.62	61.16	29.27	6.75		2.51	72.59	11.25
5	4.20	90.54	59.76	6.21	90.45	81.89	15.24		6.58	78.63	5.04
6	20.57	66.13	2.00	99.44	49.79	99.33	99.20		21.68	97.83	29.40
7	15.84	64.03	0.54	10.33	1.28	77.23	8.38		2.08	45.56	1.68

细胞群	CD34	CD38	HLA-DR	CD3	CD13	CD33	CD15	CD9	CD10	CD19	CD22	cCD79a	cMPO	TdT
原始细胞群1	9.86	78.22	84.01	38.59	52.61	40.60	3.70	72.28	32.13	93.53	78.11	79.26	40.19	31.04
原始细胞群2	19.06	95.87	63.86	43.92	81.84	88.68	53.12	52.98	30.96	73.39	46.14	34.29	69.01	19.43

细胞群	CD34	CD38	HLA-DR	CD3	CD13	CD33	CD15	CD9	CD10	CD19	CD22	cCD79a	cMPO	TdT
原始细胞群1	9.86	78.22	84.01	38.59	52.61	40.60	3.70	72.28	32.13	93.53	78.11	79.26	40.19	31.04
原始细胞群2	19.06	95.87	63.86	43.92	81.84	88.68	53.12	52.98	30.96	73.39	46.14	34.29	69.01	19.43

病例	融合基因	染色体
1	BCR/ABL1（p190）	46,XY,t（9：22）（q34：q11）^[20]
2	BCR/ABL1（p190）	47-49,XX,-21,+2-4mar[cp12]/46,XX^[8]
3	BCR/ABL1（p210）	46,XY^[20]
4	…	46,XY,t（9;22）（q34：q11）^[10]
5	BCR/ABL1（p210）	45,XX,-7,t（9;22）（q34;q11）^[20]
6	BCR/ABL1（p210）	46,XY^[20]
7	EVI1	46,XX,t（11;19）（q23;p13）^[20]
8	BCR/ABL1（p210）	46,XY,t（9;22）（q34：q11）^[10]
9	BCR/ABL1（p210）	45,XX,-7,t（9：22）（q34：q11）^[20]

Bone marrow cell morphology and immuno-phenotype characteristics of MPAL by flow cytometry

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