Cell morphology and immunophenotype in 12 patients of acute bilineage leukemia

doi:10.3969/j.issn.1673-8640.2015.05.009

Abstract

Abstract: Objective To investigate the relationship between cell morphology and immunophenotype of acute bilineage leukemia(aBLL). Methods A total of 12 aBLL patients were enrolled, and the marrow cell morphology and histochemistry staining were performed. The immunophenotype was detected, including HLA-DR, CD34, MPO, CD13, CD33, CD11b, CD117, CD14, CD64, CD3, CD4, CD7, CD10, CD19, CD20, CD22 and CD79a. Results There were not only some differences but also complementaries between FAB and immunophenotype classification of aBLL. Of the 12 aBLL patients, 6 cases were diagnosed by cell morphology as acute myeloid leukemia (AML) [M2(4 cases) and M1(2 cases)], 2 cases as acute mixed lineage leukemia (AMLL) and 4 cases as ALL-L2, respectively. The 12 cases were diagnosed by immunophenotype classification as aBLL, while 10 cases were myeloid-B lymphocyte mix (My-B), and 2 cases were myeloid-T lymphocyte mix(My-T). Conclusions aBLL is a rare type of leukemia. The complementarity between immunophenotype and cell morphology can effectively reduce the misdiagnosis and missed diagnosis of aBLL.

Key words: Immunophenotype, Acute bilineage leukemia, Cell morphology

CLC Number:

R446.11

FAN Liquan, CHEN Weiqin, WANG Jianbiao, SHENG Yan, WU Jing. Cell morphology and immunophenotype in 12 patients of acute bilineage leukemia[J]. Laboratory Medicine, 2015, 30(5): 446-449.

Figures/Tables 5

References 9

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积分	髓系	B-淋巴细胞系	T-淋巴细胞系
2	MPO	CD79a、cIgM、cCD22	CD3(m/cyt)、TCR
1	CD117、CD13、CD33、CD65	CD19、CD10、CD20	CD2、CD5、CD8、CD10
0.5	CD14、CD15、CD64	TdT、CD24	TdT、CD7、CD1a

积分	髓系	B-淋巴细胞系	T-淋巴细胞系
2	MPO	CD79a、cIgM、cCD22	CD3(m/cyt)、TCR
1	CD117、CD13、CD33、CD65	CD19、CD10、CD20	CD2、CD5、CD8、CD10
0.5	CD14、CD15、CD64	TdT、CD24	TdT、CD7、CD1a

病例	FAB分型	系列积分			骨髓幼稚细胞	POX
病例	FAB分型	髓系			B系	T系
1	AML-M2	4	5	0	0.820	(+)19%
2	AML-M2	4.5	5	0	0.790	(+)21%
3	AML-M2	4.5	5	0	0.850	(+)30%
4	AML-M2	4	5	0	0.865	(+)29%
5	AML-M1	5	0	2.5	0.910	(+)17%
6	AML-M1	4	0	2.5	0.950	(+)15%
7	AMLL	4	6	0	0.770	(+)11%
8	AMLL	4	6	0	0.830	(+)10%
9	ALL-L2	4	6	0	0.775	(-)100%
10	ALL-L2	4	4	0	0.880	(-)100%
11	ALL-L2	4	6	0	0.895	(-)100%
12	ALL-L2	4	6	0	0.840	(-)100%

病例	FAB分型	系列积分			骨髓幼稚细胞	POX
病例	FAB分型	髓系			B系	T系
1	AML-M2	4	5	0	0.820	(+)19%
2	AML-M2	4.5	5	0	0.790	(+)21%
3	AML-M2	4.5	5	0	0.850	(+)30%
4	AML-M2	4	5	0	0.865	(+)29%
5	AML-M1	5	0	2.5	0.910	(+)17%
6	AML-M1	4	0	2.5	0.950	(+)15%
7	AMLL	4	6	0	0.770	(+)11%
8	AMLL	4	6	0	0.830	(+)10%
9	ALL-L2	4	6	0	0.775	(-)100%
10	ALL-L2	4	4	0	0.880	(-)100%
11	ALL-L2	4	6	0	0.895	(-)100%
12	ALL-L2	4	6	0	0.840	(-)100%

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