Abstract:

Objective To investigate serum immunoglobulin（Ig） and other factors on the effect of blood separating in separator gel tubes. Methods A total of 221 multiple myeloma（MM） patients were enrolled（149 cases of IgG type,65 cases of IgA type and 7 cases of IgM type）. According to Ig levels,IgG type was classified into IgG≤47 g/L group（group A,84 cases） and IgG>47 g/L group（group B,65 cases）,IgA type was classified into IgA≤38 g/L group（group C,39 cases） and IgA>38 g/L group（group D,26 cases）,and IgM type was IgM≤63 g/L group（group E,7 cases）. According to total protein（TP） levels,50 patients with autoimmune disease were classified into TP≤90 g/L group（group F,27 cases） and TP>90 g/L group（group G,23 cases）. According to hematocrit（HCT） levels,55 patients with anemia were classified into HCT≤30% group（group H,26 cases） and HCT>30% group（group I,29 cases）. Fasting venous blood was collected in 2 kinds of separator gel tubes. Centrifugation occurred after complete solidification,and then blood separating effects were observed. Results For two kinds of separator gel tubes of group B and group D,there were 4 layers,including serum,red blood cell（RBC）,separator gel and RBC from top to bottom. The top serum was rare,following RBC increased significantly,and bottom RBC was rare. For two kinds of separator gel tubes of group A,C,E,F,G,H and I,there were 3 layers,including serum,separator gel and RBC from top to bottom. Serum was rich. Conclusions Blood separating in separator gel tubes is influenced by many factors,such as the levels of Ig,TP and HCT,and MM patients with increasing levels of Ig and TP and decreasing level of HCT are inappropriate for using separator gel tubes.

Key words: Immunoglobulin, Separator gel tube, Multiple myeloma