Abstract: Objective To analyze the drug resistance and gene of Enterococcus faecalis and Enterococcus faecium isolated from the midstream urine of patients with urinary tract infection in Antu Hospital, and to provide the reference for clinical medication. Methods Enterococcus faecalis (23 isolates) and Enterococcus faecium (18 isolates) were isolated and collected from the midstream urine of patients with urinary tract infection. The bacteria were identified by API, and drug sensitivity test was done by Kirby-Bauer disk diffusion method. Drug resistance genes were also detected by polymerase chain reaction(PCR). Results The positive rates of tetM, ermB, aac(6′)/aph(2′), ant(6)-Ⅰ and aph(3′)-Ⅲ genes were 65.2%, 82.6%, 100.0%, 100.0% and 100.0% in the 23 isolates of Enterococcus faecalis. In the 18 isolates of Enterococcus faecium, the positive rates of tetM, ermB, aac(6′)/aph(2′), ant(6)-Ⅰ and aph(3′)-Ⅲ genes were 55.5%, 55.5%, 100.0%, 94.4% and 100.0%. The drug resistance rates of Enterococcus faecalis to panicillin, tetracyline, ciprofloxacin, levofloxacin and erythomycin were >50.0%. The drug resistance rates of Enterococcus faecium to penicillin, ampicillin, ciprofloxacin, levofloxacin and erythomycin were 100.0%. Results The positive rate of drug resistance genes is high in Enterococcus isolated from clinic, which has a high multi-drug resistance.

Key words: Enterococcus faecalis, Enterococcus faecium, Drug resistance, Drug resistance gene, Antibacterial drug