关键词: 人类免疫缺陷病毒, 丙型肝炎病毒, 核酸检测, 外周血单个核细胞, CD4⁺T细胞计数

Abstract:

Objective　To investigate the ratio of hepatitis C virus（HCV） co-infected with human immunodeficiency virus（HIV）-1 and the impact of HCV co-infection on CD4⁺ T cell counts in HIV-1 infected patients. Methods　HCV-Ab was detected by enzyme-linked immunosorbent assay（ELISA）， and HCV-RNA was determined by polymerase chain reaction（PCR） with serum and peripheral blood mononuclear cell（PBMC）. Results　Among 234 HIV-1 infected patients， the infection through sex accounted for 83.7%（male gay sex for 48.7% and heterosexual contact for 35.0%）， drug addiction for 4.2%， inpatient for 2.6% and maternal-neonatal transmission for 2.1%. The percentage of HCV-Ab positive was 12.8%（30/234）， that of serum HCV-RNA was 11.5%（27/234）， which 26 cases were HCV-Ab positive and 1 case was HCV-Ab negative， while the serum HCV-RNA content was 3.60×10³copies/mL， and 14 cases were HCV-RNA positive in PBMC accounting for 6.0%， thus the ratio of HCV co-infection was 13.2% as a total. The average counts of CD4⁺ T cell among HIV-1 HCV infection and single HIV-1 infection were 322 cells/mL and 477 cells/mL. Conclusions　The ratio of HIV-1 HCV co-infection was 13.2% in the South of Anhui， and the determination of HCV during screening HIV-1 infection may help with the early diagnosis and treatment of HCV， and the detection of HCV-RNA with PBMC should be taken attention.

Key words: Human immunodeficiency virus 1, Hepatitis C virus, RNA determination, Peripheral blood mononuclear cell, CD4⁺ T cell count