关键词: 人类免疫缺陷病毒, 抗体, 免疫印迹法, 转归

Abstract:

Objective To investigate the characteristics and outcomes of patients with indeterminate western-blot results of human immunodeficiency virus（HIV）-1 antibody. Methods Totally,75 patients with indeterminate western-blot results of HIV-1 antibody were enrolled. The general data,screening test results [enzyme-linked immunosorbent assay（ELISA）and colloid se method],western-blot results and the outcomes of follow-up were collected. Results In the 75 cases,53 cases were followed up,and the follow-up time was 10-721 d. The difference of positive conversion rate between males and females had statistical significance（P<0.05）. Among the 53 cases,the positive consistency rate of the 2 screening tests was 100%（45 cases）. Using receiver operating characteristic（ROC） curve analysis,the optimal cut-off value of ELISA S/CO value was 4.995,the area under curve（AUC） was 0.969,the sensitivity was 84.8%,and the specificity was 100.0%. With the increase of ELISA S/CO values,the western-blot bands turned into positive correspondingly,and the occurrence rates of env gp41,gag p66 and p51 were also increased correspondingly. Conclusions The collaborative analysis of colloid se method,ELISA S/CO value and western-blot band will be helpful for the determination of indeterminate HIV-1 antibody.

Key words: Human immunodeficiency virus, Antibody, Western-blot, Outcome