关键词: 乙型肝炎病毒, 基因型, 脱氧核糖核酸, 聚合酶链反应-反向点杂交法

Abstract:

Objective　To understand the distribution of genotypes in Tongling area， to analyze the relationship of different genotypes with liver function damage， virus  replication and serum markers， and to investigate the clinical significance of different genotypes. Methods　Polymerase chain reaction（PCR）-reverse dot blot RDB） was used to determine hepatitis B virus（HBV） genotypes， HBV load， serum markers of hepatitis B and liver function among 228 patients infected with HBV in Tongling area. The correlation analysis was performed with other parameters. Results　In 228 serum samples， 226 samples were successfully genotyped， including 54.4% B type（124/228）， 37.3% C type（85/228） and 7.5% B/C hybrid type（17/228）， and 2 cases（0.09%） were not detected. Differences between different HBV genotypes and age， e antigen（HBeAg）， anti-e antigen antibody（anti-HBe） and liver function had no statistical significance（P＞0.05）. The C type HBV load was significantly higher than those of B type and B/C hubrid type（P＜0.01）. Conclusions　The distribution of HBV genotypes in Tongling area is dominated by B type， followed by C type and a small amount of B/C hybrid type. C type infected patients′ HBV load is significantly higher than that of B type. The liver damage of patients with C type is more serious than that of B type， which prompts that HBV genotypes may influence on in vivo HBV replication and may cause chronic liver disease of different clinical types.

Key words: Hepatitis B virus, Genotype, DNA, Polymerase chain reaction-reverse dot blot