关键词: 慢性粒细胞白血病, 核酸适配子, 检测方法, 诊断价值

Abstract: Objective To evaluate the significance of K562 cell ssDNA aptamer detection method for the clinical diagnosis of chronic myeloid leukemia(CML).Methods A total of 5 mL blood samples of 18 patients with CML, 22 patients with other leukemia and 20 healthy subjects were collected. The granulocytes were separated, and aptamer detection method was established for the determination of fluorescence intensity. The receiver operating characteristics (ROC) curve was drawn. Results The fluorescence intensities were 492.26±41.67 in CML group, 466.86±33.23 in other leukemia group and 469.33±37.13 in healthy control group, and the results showed no statistical significance (P=0.078 and P=0.243). There was no statistical significance between other leukemia group and healthy control group (P=0.835). The area under ROC curve was 0.702, the optimal positive threshold was 475.13, the sensitivity was 83.33%, the specificity was 54.76%, the positive predictive value was 44.11%, and the negative predictive value was 88.46%. Conclusions The aptamer detection method has a low accuracy in the clinical diagnosis of CML, and it has some limitations for clinical application.

Key words: Chronic myeloid leukemia, Aptamer, Detection method, Diagnosis significance