检验医学 ›› 2020, Vol. 35 ›› Issue (10): 971-973.DOI: 10.3969/j.issn.1673-8640.2020.10.002

• 碳青霉烯类耐药肠杆菌科细菌临床检测与治疗方案的发展及应用专题 • 上一篇    下一篇

碳青霉烯类耐药肠杆菌科细菌的检测方法

周宏伟, 胡燕燕, 张嵘()   

  1. 浙江大学医学院附属第二医院检验科,浙江 杭州 310009
  • 收稿日期:2019-11-21 出版日期:2020-10-30 发布日期:2020-11-12
  • 作者简介:null
    作者简介:周宏伟,男,1980年生,博士,副主任技师,主要从事尿道致病性大肠埃希菌致病机制研究。

Determination methods for carbapenem-resistant Enterobacteriaceae

ZHOU Hongwei, HU Yanyan, ZHANG Rong()   

  1. Department of Clinical Laboratory,the Second Affiliated Hospital of Zhejiang University School of Medicine,Hangzhou 310009,Zhejiang,China
  • Received:2019-11-21 Online:2020-10-30 Published:2020-11-12

摘要:

由碳青霉烯类耐药肠杆菌科细菌(CRE)引起的感染给临床治疗带来了巨大挑战。及时、准确地鉴别出产何种碳青霉烯酶,对指导临床抗感染治疗有至关重要的意义。目前,碳青霉烯酶的检测方法主要为表型筛选、基因检测和基于基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术的检测方法。文章对CRE的各类检测方法进行综述。

关键词: 碳青霉烯类耐药, 肠杆菌科细菌, 检测方法

Abstract:

The infection caused by carbapenem-resistant Enterobacteriaceae(CRE) brings a challenge for clinical treatment,and timely and accurately identifying carbapenemase plays a role in guiding clinical antibiotic application. At present,the determination methods of carbapenemase include phenotypic screening,gene determination and the methods based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS). This review focuses on various determination methods for CRE.

Key words: Carbapenem resistance, Enterobacteriaceae, Determination method

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