关键词: 光滑念珠菌, 基因分型, 多位点序列分析, 微卫星多态性分析

Abstract:

Objective To evaluate the application significance of microsatellite polymorphism in genotyping of Candida glabrata. Methods From January 2011 to December 2012, 59 isolates of Candida glabrata were collected from Renji Hospital and East Hospital. All the 59 isolates were typed by microsatellite polymorphism and multilocus sequence typing (MLST). The results and discrimination of the 2 genotyping methods were compared. Results By MLST, the 59 isolates belonged to 6 clone sequences, including 43 isolates with ST-7, 7 isolates with ST-10, 3 isolates with ST-15, 3 isolates with ST-55, 2 isolates with ST-3 and 1 isolate with ST-43, and the index of discriminatory power (DP) was 0.456. By microsatellite polymorphism, the 59 isolates were classified into 10 genotypes. There were 25 isolates with type A, 10 isolates with type B, 8 isolates with type C, 6 isolates with type D, 3 isolates with type E, 2 isolates with type F, 2 isolates with type G, 1 isolate with type H, 1 isolate with type I and 1 isolate with type J, and the DP was 0.770. Conclusions Microsatellite polymorphism is a simple and rapid method for molecular typing with higher discrimination than MLST. Therefore, microsatellite polymorphism can be the preferred choice in clinical laboratories.

Key words: Candida glabrata, Genotype, Multilocus sequence typing, Microsatellite polymorphism