GeneXpert实时荧光定量PCR快速检测艰难梭菌的临床评估

doi:10.3969/j.issn.1673-8640.2014.06.003

检验医学 ›› 2014, Vol. 29 ›› Issue (6): 590-592.DOI: 10.3969/j.issn.1673-8640.2014.06.003

GeneXpert实时荧光定量PCR快速检测艰难梭菌的临床评估

收稿日期:2013-11-09 出版日期:2014-06-30 发布日期:2014-06-23
通讯作者: 韩立中,联系电话:021-64370045-600632
作者简介:陈旭,男,1988年生, 硕士,主要从事微生物学研究。
基金资助:
卫生公益性行业科研专项(201002021)

Clinical evaluation of GeneXpert real-time fluorescence quantitation PCR in rapid detection of Clostridium difficile

1. Department of Clinical Microbiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China;
2. Department of Clinical Laboratory, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

Received:2013-11-09 Online:2014-06-30 Published:2014-06-23

摘要/Abstract

摘要：

目的对GeneXpert实时荧光定量聚合酶链反应(PCR)在快速检测临床粪便标本中艰难梭菌的应用进行评估。方法采用双拭子蘸取临床未成形粪便标本,一支拭子用于GeneXpert实时荧光定量PCR检测艰难梭菌毒素基因tcdB,另一支用于常规厌氧菌培养检测;对GeneXpert实时荧光定量PCR检测结果与常规厌氧菌培养结果的一致性进行统计学分析,并计算GeneXpert实时荧光定量PCR的敏感性、特异性、阳性预测值和阴性预测值等参数。结果临床收集到141例未成形粪便标本,GeneXpert实时荧光定量PCR检出艰难梭菌毒素基因tcdB阳性42例,其中常规厌氧菌培养阳性34例,两者一致性较好(Kappa=0.775 0,P<0.01),GeneXpert实时荧光定量PCR的敏感性、特异性、阳性预测值和阴性预测值分别为87.2%、92.2%、81.0%和94.9%。结论 GeneXpert实时荧光定量PCR直接检测粪便标本中的艰难梭菌具有检测快速、操作简便等优点,有重要的临床应用价值。

关键词: 艰难梭菌, 快速检测, 实时荧光定量聚合酶链反应

Abstract:

Objective To evaluate the clinical application of GeneXpert real-time fluorescence quantitation polymerase chain reaction (PCR) in rapid detection of Clostridium difficile from stool. Methods Two-head swab was dipped in clinical stool specimens, one-head swab was used for GeneXpert real-time fluorescence quantitation PCR in detecting toxity gene tcdB , and the other was used for anaerobic culture. The results of these 2 methods were compared and analyzed statistically, and the sensitivity, specificity, positive predictive value and negative predictive value of GeneXpert real-time fluorescence quantitation PCR were calculated. Results Totally 141 clinical stool specimens were collected, and GeneXpert real-time fluorescence quantitation PCR revealed that Clostridium difficile was positive in 42 cases, among which Clostridium difficile was cultured in 34 cases. GeneXpert real-time fluorescence quantitation PCR was well consistent with the culture method (Kappa=0.775 0, P<0.01), and the sensitivity, specificity, positive predictive value and negative predictive value of GeneXpert real-time fluorescence quantitation PCR were 87.2%, 92.2%,81.0% and 94.9%, respectively. Conclusions GeneXpert real-time fluorescence quantitation PCR could detect Clostridium difficile from stool specimens directly and accurately, and it has a prospective potential for clinical application.

Key words: Clostridium difficile, Rapid detection, Real-time fluorescence quantitation polymerase chain reaction

中图分类号:

R446.5

陈旭,董丹凤,韩立中,倪语星. GeneXpert实时荧光定量PCR快速检测艰难梭菌的临床评估[J]. 检验医学, 2014, 29(6): 590-592.

CHEN Xu, DONG Danfeng, HAN Lizhong, NI Yuxing. Clinical evaluation of GeneXpert real-time fluorescence quantitation PCR in rapid detection of Clostridium difficile[J]. , 2014, 29(6): 590-592.

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GeneXpert实时荧光定量PCR快速检测艰难梭菌的临床评估

Clinical evaluation of GeneXpert real-time fluorescence quantitation PCR in rapid detection of Clostridium difficile

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