miRNA“封闭海绵”的构建与应用

doi:10.3969/j.issn.1673-8640.2014.02.021

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 178-181.DOI: 10.3969/j.issn.1673-8640.2014.02.021

miRNA“封闭海绵”的构建与应用

李凤综述, 李智审校

同济大学附属第十人民医院检验科, 上海 200072

收稿日期:2012-12-13 出版日期:2014-02-20 发布日期:2014-02-20
通讯作者: 李智, 联系电话:021-65690520-333
作者简介:李凤, 女, 1988年生, 主要从事恶性肿瘤发病机制、早期诊断及其检测方法学研究。
基金资助:
国家自然基金面上项目 (81171883)

Establishment and application of miRNA sponges

LI Feng, LI Zhi

Department of Clinical Laboratory, Tenth People′s Hospital of Tongji Hospital, Shanghai 200072, China

Received:2012-12-13 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要： 微小RNA是一类非编码的小分子RNA, 通过参与基因的转录后水平表达调控, 调控机体的多种生理与病理进程。微小RNA(microRNA, miRNA)“封闭海绵”是一类可以封闭特定miRNA的物质。“封闭海绵”通过特定位点与mRNA的3′UTR区结合来发挥封闭微小RNA的作用。现有研究表明“封闭海绵”作为一种新型的研究miRNA的方法在生物体内、外功能丧失研究中均优于目前的反义寡核苷酸抑制剂和基因敲除方法。随着越来越多非编码RNA的发现, “封闭海绵”在研究其功能上有着不可或缺的作用。本研究对近年在“封闭海绵”的构建与应用方面的研究作一综述。

关键词: 微小RNA, “封闭海绵”, 基因调控

Abstract: MicroRNA(miRNA) is a kind of non-coding small RNA which participates in post-transcriptional expression modulation and involves in various kinds of physiological and pathologicalprocesses. miRNA sponges can sequester specific miRNA. miRNA sponges can bind to specific sites in the 3′untranslated region (UTR) of mRNA to sequester miRNA. Compared with antisense oligonucleotide inhibitors and genetic knockouts, miRNA sponges are valuable tools for miRNA loss-of-function studies in vitro and in vivo.With more and more non-coding RNAs were discovered, miRNA sponge has a indispensable role in the research of its function. This research will discuss the establishment and application of miRNA sponges.

Key words: MicroRNA, Sponge, Gene expression modulation

中图分类号:

Q503

李凤综述, 李智审校. miRNA“封闭海绵”的构建与应用[J]. 检验医学, 2014, 29(2): 178-181.

LI Feng, LI Zhi. Establishment and application of miRNA sponges[J]. , 2014, 29(2): 178-181.

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miRNA“封闭海绵”的构建与应用

Establishment and application of miRNA sponges

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