血清miR-150-5p和LTBP-2检测在急性脑梗死患者病情评估中的价值

doi:10.3969/j.issn.1673-8640.2023.11.014

摘要/Abstract

摘要：

目的探讨血清miR-150-5p和潜伏转化生长因子结合蛋白（LTBP）-2相对表达量在急性脑梗死（ACI）患者病情评估中的价值。方法选取2018年5月—2021年2月义乌復元私立医院ACI患者121例（ACI组）。根据发病48~72 h内脑梗死体积分为小梗死组、中梗死组、大梗死组；根据美国国立卫生研究院卒中量表（NIHSS）评分将ACI患者分为轻度组、中度组、重度组。以体检健康者128名作为正常对照组。检测所有研究对象血清miR-150-5p和LTBP-2 mRNA的表达情况。采用Pearson相关分析评估ACI患者miR-150-5p与LTBP-2 mRNA的相关性。采用Logistic回归分析评估发生ACI的危险因素。结果与正常对照组比较，ACI组血清miR-150-5p相对表达量显著降低（P<0.001），而LTBP-2 mRNA相对表达量显著升高（P<0.001）。小梗死组、中梗死组和大梗死组血清miR-150-5p相对表达量依次降低（P<0.001），LTBP-2 mRNA相对表达量依次升高（P<0.001）。轻度组、中度组和重度组血清miR-150-5p相对表达量依次降低（P<0.001），LTBP-2mRNA相对表达量依次升高（P<0.001）。Targetscan网站预测结果显示，LTBP-2是miR-150-5p潜在的靶基因。Pearson相关分析结果显示，ACI组血清miR-150-5p表达与LTBP-2 mRNA表达呈负相关（r=-0.346，P<0.001）。多因素Logistic回归分析结果显示，miR-150-5p相对表达量降低、LTBP-2 mRNA相对表达量升高是ACI发生的危险因素[比值比（OR）值分别为0.563、1.698，95%可信区间（CI）分别为0.445~0.712、1.342~2.148]。结论 ACI患者血清miR-150-5p和LTBP-2表达与疾病严重程度有关，或可作为ACI病情评估的指标。

关键词: 微小RNA-150-5p, 潜伏转化生长因子结合蛋白2, 急性脑梗死

Abstract:

Objective To investigate the clinical roles of relative expression levels of serum miR-150-5p and latent transforming growth factor-beta binding protein（LTBP）-2 in evaluating the condition of acute cerebral infarction（ACI）. Methods Totally，121 ACI patients（ACI group）from Yiwu Fuyuan Private Hospital from May 2018 to February 2021 were enrolled and classified into small infarction group，medium infarction group and large infarction group based on the volume of cerebral infarction within 48-72 h of onset. According to the National Institute of Health Stroke Scale（NIHSS） score，ACI patients were classified into mild group，moderate group and severe group. A total of 128 healthy subjects were enrolled as healthy control group. The relative expression levels of miR-150-5p and LTBP-2 mRNA in serum were determined. Pearson correlation analysis was used to evaluate the correlation between miR-150-5p and LTBP-2 mRNA in ACI patients. Logistic regression analysis was used to evaluate the risk factors of ACI occurrence. Results Compared with healthy control group，the relative expression level of miR-150-5p in serum of ACI group was decreased（P<0.001），while the relative expression level of LTBP-2 mRNA was increased（P<0.001）. The relative expression levels of miR-150-5p in serum of small infarction group，medium infarction group and large infarction group were decreased in turn（P<0.001），while the relative expression levels of LTBP-2 mRNA were increased（P<0.001）. The relative expression levels of miR-150-5p in serum of mild，moderate and severe groups were decreased in turn（P<0.001），while the relative expression levels of LTBP-2 mRNA were increased（P<0.001）. The prediction results of Targetscan website showed that LTBP-2 was a potential target gene for miR-150-5p. Pearson correlation analysis showed a negative correlation between serum miR-150-5p and LTBP-2 mRNA expression levels in ACI group（r=-0.346，P<0.001）. The results of multivariate Logistic regression analysis showed that decreased expression level of miR-150-5p and increased expression level of LTBP-2 mRNA were risk factors for the occurrence of ACI [odds ratios（OR） were 0.563 and 1.698，95% confidence intervals（CI）were 0.445-0.712 and 1.342-2.148，respectively]. Conclusions The relative expression levels of miR-150-5p and LTBP-2 in serum of ACI patients are related to disease severity，and they can be used as the indicators for evaluating the condition of ACI.

Key words: MicroRNA-150-5p, Latent transforming growth factor-beta binding protein-2, Acute cerebral infarction

中图分类号:

R446.7

薛亚泽, 何劲, 唐绍军. 血清miR-150-5p和LTBP-2检测在急性脑梗死患者病情评估中的价值[J]. 检验医学, 2023, 38(11): 1082-1086.

XUE Yaze, HE Jin, TANG Shaojun. Roles of serum miR-150-5p and LTBP-2 determinations in evaluating the condition of acute cerebral infarction patients[J]. Laboratory Medicine, 2023, 38(11): 1082-1086.

图/表 8

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基因名称	上游引物（5'~3'）	下游引物（5'~3'）
miR-150-5p	CAGCCAGAGTTAGCACAATAGG	CTGTTGTTCCCGTCGGAGTT
U6	AACGCTTCACGATTTGCGT	CTCGCTTCGGCAGCACA
β-actin	TGACGAGACCTCTATGCCGACTC	CGGACTCATCGTACTCCTGCT
LTBP-2	GGGCACCGCACCACCTACACG	TCATCACACTCATTCACATCTACG

基因名称	上游引物（5'~3'）	下游引物（5'~3'）
miR-150-5p	CAGCCAGAGTTAGCACAATAGG	CTGTTGTTCCCGTCGGAGTT
U6	AACGCTTCACGATTTGCGT	CTCGCTTCGGCAGCACA
β-actin	TGACGAGACCTCTATGCCGACTC	CGGACTCATCGTACTCCTGCT
LTBP-2	GGGCACCGCACCACCTACACG	TCATCACACTCATTCACATCTACG

组别	例数	BMI/（kg/m²）	饮酒史/[例（%）]	吸烟史/[例（%）]	高血压史/[例（%）]
正常对照组	128	23.14±2.28	40（31.25）	37（28.91）	29（22.66）
ACI组	121	22.68±2.47	34（28.10）	35（28.93）	25（20.66）
统计值		1.528	0.296	0.019	0.052
P值		0.128	0.587	0.891	0.820

组别	例数	BMI/（kg/m²）	饮酒史/[例（%）]	吸烟史/[例（%）]	高血压史/[例（%）]
正常对照组	128	23.14±2.28	40（31.25）	37（28.91）	29（22.66）
ACI组	121	22.68±2.47	34（28.10）	35（28.93）	25（20.66）
统计值		1.528	0.296	0.019	0.052
P值		0.128	0.587	0.891	0.820

组别	例数	miR-150-5p	LTBP-2 mRNA
正常对照组	128	1.00±0.18	1.00±0.14
ACI组	121	0.76±0.20	1.30±0.32
t值		9.963	9.673
P值		<0.001	<0.001