miR-335和Fra-1在乳腺癌中的表达及相互关系

doi:10.3969/j.issn.1673-8640.2023.02.008

摘要/Abstract

摘要：

目的探讨微小RNA-335（miR-335）和Fos相关抗原1（Fra-1）在乳腺癌组织和乳腺癌细胞中的表达，以及二者的关系。方法收集41例乳腺癌患者癌组织和对应的癌旁组织（2 cm<距癌组织边缘<3 cm），检测不同组织、乳腺癌细胞系（MDA-231和MCF-7）和正常乳腺细胞系（MCF-10A）中miR-335、Fra-1 mRNA和Fra-1蛋白表达。向乳腺癌细胞系（MDA-231和MCF-7）分别转染含miR-335的质粒（miR-335 mimic）和对照质粒（mimic control），分析过表达的miR-335对Fra-1 mRNA和Fra-1蛋白表达的影响。采用Pearson相关分析评估miR-335与Fra-1的相关性。结果与正常乳腺细胞系（MCF-10A）比较，乳腺癌细胞系（MCF-7和MDA-231）中miR-335 mRNA表达降低（P<0.05），Fra-1 mRNA和Fra-1蛋白表达均升高（P<0.05）。与癌旁组织比较，乳腺癌组织中miR-335表达降低（P<0.05），Fra-1 mRNA和Fra-1蛋白表达均升高（P<0.05）。乳腺癌组织中Fra-1阳性例数显著多于癌旁组织（P<0.05）。与各自的对照比较，MCF-7细胞和MDA-231细胞转染miR-335 mimic后，miR-335表达上调（P<0.05），Fra-1 mRNA和Fra-1蛋白表达下调（P<0.05）。Pearson相关分析结果显示，miR-335 与Fra-1 mRNA呈负相关（r=-0.830 4，P<0.05）。结论在乳腺癌组织和乳腺癌细胞系中，miR-335呈低表达，Fra-1呈高表达。过表达miR-335可降低乳腺癌细胞中Fra-1的表达。

关键词: 微小RNA-335, Fos相关抗原1, 乳腺癌

Abstract:

Objective To determine the expressions of microRNA-335（miR-335）and Fos related antigen-1（Fra-1）in breast cancer tissues and cells，and to investigate the relationship between them. Methods The breast cancer tissues and corresponding paracancerous tissues from 41 patients were collected（>2 cm and <3 cm away from the lesions）. The expressions of miR-335，Fra-1 mRNA and Fra-1 protein were determined in different tissues，breast cancer cell lines（MDA-231 and MCF-7） and normal breast cell line（MCF-10A）. Breast cancer cell lines（MDA-231 and MCF-7） were transfected with miR-335 containing plasmid（miR-335 mimic） and control plasmid（mimic control），and the effect of miR-335 overexpression on Fra-1 mRNA and Fra-1 protein expressions was analyzed. Pearson correlation analysis was used to evaluate the correlation between miR-335 and Fra-1. Results Compared with the normal breast cell line（MCF-10A），the expression of miR-335 in breast cancer cell lines（MCF-7 and MDA-231） was decreased（P<0.05），and the expressions of Fra-1 mRNA and Fra-1 protein were increased（P<0.05）. Compared with tumor-adjacent tissues，the expression of miR-335 in breast cancer tissues was decreased（P<0.05），and the expressions of Fra-1 mRNA and Fra-1 protein were increased（P<0.05）. The positive expression of Fra-1 in breast cancer tissues was higher than that in adjacent tissues（P<0.05）. Compared with their respective control groups，the expressions of miR-335 in MCF-7 cells and MDA-231 cells transfected with miR-335 mimic were up-regulated（P<0.05），and the expressions of Fra-1 mRNA and Fra-1 protein were down-regulated（P<0.05）. Pearson correlation analysis showed that miR-335 was negatively correlated with Fra-1 mRNA（r=-0.830 4，P<0.05）. Conclusions The expression of miR-335 decreases，and Fra-1 increases in breast cancer tissues and cell lines. Overexpression of miR-335 reduces the expression of Fra-1 in breast cancer cells.

Key words: MicroRNA-335, Fos related antigen-1, Breast cancer

中图分类号:

R446.1

刘冲, 张静, 李胜, 赵奇. miR-335和Fra-1在乳腺癌中的表达及相互关系[J]. 检验医学, 2023, 38(2): 143-147.

LIU Chong, ZHANG Jing, LI Sheng, ZHAO Qi. Expressions and correlation of miR-335 and Fra-1 in breast cancer[J]. Laboratory Medicine, 2023, 38(2): 143-147.

图/表 5

图1 乳腺癌细胞系（MCF-7、MDA-231）与正常乳腺细胞系（MCF-10A）miR-335、Fra-1 mRNA和Fra-1蛋白表达比较注：（a）MCF-10A细胞、MCF-7细胞、MDA-231细胞miR-335相对表达量比较；（b）MCF-10A细胞、MCF-7细胞、MDA-231细胞Fra-1 mRNA相对表达量比较；（c）MCF-10A细胞、MCF-7细胞、MDA-231细胞Fra-1蛋白表达的免疫印迹图；（d）MCF-10A细胞、MCF-7细胞、MDA-231细胞Fra-1蛋白相对表达量比较；与MCF-10A细胞比较，*P<0.05。

图2 乳腺癌组织和癌旁组织miR-335、Fra-1 mRNA和Fra-1蛋白表达的比较注：（a）乳腺癌组织与癌旁组织miR-335表达的比较；（b）乳腺癌组织与癌旁组织Fra-1 mRNA表达的比较；（c）乳腺癌组织和癌旁组织Fra-1蛋白表达免疫印迹图；（d）乳腺癌组织与癌旁组织Fra-1蛋白表达的比较；（e）乳腺癌组织免疫组化结果；（f）癌旁组织免疫组化结果；与癌旁组织比较，*P<0.05。

表1 乳腺癌组织和癌旁组织Fra-1染色结果比较例

组织类型	阳性	弱阳性	阴性
癌组织	25	13	3
癌旁组织	4	6	31
χ²值		40.845
P值		<0.001

图3 MCF-7细胞、MDA-231细胞转染miR-335 mimic后miR-335、Fra-1 mRNA和Fra-1蛋白表达注：（a）MCF-7细胞和MDA-231细胞miR-335相对表达量比较；对照；转染miR-335 mimic；（b）MCF-7细胞和MDA-231细胞Fra-1 mRNA相对表达量比较；对照；转染miR-335 mimic；（c）MCF-7细胞Fra-1蛋白表达情况；（d）MDA-231细胞Fra-1蛋白表达情况；与对照比较，*P<0.05。

图4 乳腺癌细胞中miR-335与Fra-1 mRNA表达的相关性注：r=-0.830 4，P<0.05。

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