检验医学 ›› 2014, Vol. 29 ›› Issue (2): 100-103.DOI: 10.3969/j.issn.1673-8640.2014.02.002

• 临床应用研究·论著 • 上一篇    下一篇

上海某医院2011年1至5月住院病例2 044份血及体液标本血培养结果分析

赵忆文1, 朱召芹2, 蔡金凤2, 陈海丽2, 王茉婴2, 胡芸文2, 张军2, 宰淑蓓2   

  1. 1.普陀区妇婴保健院, 上海 200060;
    2.上海市公共卫生临床中心, 上海 201508
  • 收稿日期:2013-02-12 出版日期:2014-02-20 发布日期:2014-02-20
  • 通讯作者: 宰淑蓓, 联系电话:021-65155256
  • 作者简介:赵忆文, 女, 1967年生, 技师, 主要从事微生物检验和医院感染研究。
  • 基金资助:
    国家自然科学基金青年基金项目(31200108);十二五科技重大专项(2013ZX10004-221和2013ZX10004221-004)

Analysis on the blood culture results of 2 044 blood and body fluid specimens of inpatients from a hospital in Shanghai from January to May, 2011

ZHAO Yiwen1, ZHU Zhaoqin2, CAI Jinfeng2, CHEN Haili2, WANG Moying2, HU Yunwen2, ZHANG Jun2, ZAI Shubei2.   

  1. 1. Women and Children Hospital of Shanghai Putuo District, Shanghai 200060, China; 2. Shanghai Public Health Clinical Center, Shanghai 201508, China
  • Received:2013-02-12 Online:2014-02-20 Published:2014-02-20

摘要: 目的 监测和分析上海市公共卫生临床中心血及体液标本血培养分离各种细菌的分布趋势, 评价全自动血培养仪阳性结果中的菌群类型和假阳性/假阴性情况。方法 采用法国生物梅里埃公司全自动血培养仪和细菌鉴定仪对2011年1至5月收集的2 044份血及体液标本进行培养和鉴定, 并进行结果分析。结果 不同标本的细菌阳性检出率不同, 穿刺液标本的细菌阳性检出率最高(38.10%), 其次为血液标本(30.74%)。共分离出231株细菌, 总阳性检出率为11.30%, 均为单一菌种。其中凝固酶阴性葡萄球菌最多(41.13%), 其次为真菌(18.18%), 金黄色葡萄球菌所占比例最少(0.87%)。结论 了解血及体液标本培养的菌群类型和分布情况, 对致病菌和污染菌进行综合鉴别和分析, 将有助于指导或帮助临床用药, 预防败血症的发生。

关键词: 血培养, 细菌分离和提纯, 全自动血培养仪

Abstract: Objective To analyze and monitor the distribution trend of bacterium strains in blood cultures for blood and body fluid specimens in Shanghai Public Health Clinical Center, and to evaluate the false positive / false negative detection results of automated blood culture analyzer. Methods A total of 2 044 blood culture results of different clinical blood and body fluid specimens from January to May, 2011 were collected. Specimens were cultured and identified byMéliès automated blood culture analyzer and identification board. The results were analyzed.Results Different specimens had different positive rates. The puncture fluid specimens had the highest bacterium culture positive rate (38.10%), followed by blood specimens (30.74%). The 231 bacterium strains were isolated. The total positive rate was 11.30%. Coagulase-negative Staphylococcus was the most, accounting for 41.13%, followed by fungi (18.18%) and Staphylococcus aureus (0.87%). Conclusions The bacterium identification and distribution of the blood and body fluidspecimens and the analysis of pathogens and contaminative bacteria will help for guiding the clinical treatment and prevention of sepsis.

Key words: Blood culture, Bacterium isolation and purification, Automated blood culture analyzer

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