充血性心力衰竭患者血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体检测的意义

doi:10.3969/j.issn.1673-8640.2014.02.001

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 97-99.DOI: 10.3969/j.issn.1673-8640.2014.02.001

• 临床应用研究·论著 • 下一篇

充血性心力衰竭患者血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体检测的意义

刘辰庚¹, 夏鸣², 王金玲¹, 李蕾¹, 王培昌¹

1. 首都医科大学宣武医院检验科, 北京 100053;
2. 闵行区中心医院检验科, 上海 201100

收稿日期:2013-02-04 出版日期:2014-02-20 发布日期:2014-02-20
通讯作者: 王培昌, 联系电话:010-83198688
作者简介:刘辰庚, 男, 1980年生, 博士, 主管技师, 主要从事衰老及相关疾病标志物筛选研究。

The significance of serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibody determination in patients with congestive heart failure

LIU Chengeng¹, XIA Ming², WANG Jinling¹, LI Lei¹, WANG Peichang¹

1. Department of Clinical Laboratory, Xuanwu Hospital, Capital Medical University, Beijing 100053, China; 2. Department of Clinical Laboratory, Central Hospital of Minhang District, Shanghai 201100, China

Received:2013-02-04 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要： 目的使用已建立的方法检测充血性心力衰竭(CHF)患者血清中的抗碳酸酐酶Ⅱ和Ⅲ自身抗体、抗氧化物及细胞因子水平, 初步评价其临床意义。方法选取58例3个月~1年前曾发生心肌梗死的CHF患者和58名健康对照者, 测定其血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体、红细胞沉降率(ESR)、超氧化物歧化酶(SOD)等5种抗氧化物和白细胞介素2(IL-2)等5种细胞因子的水平。结果 CHF患者血清抗碳酸酐酶Ⅱ自身抗体和ESR水平显著高于对照组(P<0.05), SOD、谷胱甘肽过氧化物酶(GPx)、过氧化氢酶(CAT)和总抗氧化能力(TAC)显著低于对照组(P<0.05), 丙二醛(MDA)水平显著高于对照组(P<0.05)。CHF患者血清的IL-2和白细胞介素17(IL-17)水平较对照组显著升高(P<0.05), 白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)和γ干扰素(IFN-γ)与对照组差异无统计学意义(P>0.05)。结论 CHF患者可能存在自身免疫的现象, IL-2、IL-17和抗氧化能力的检测可能作为判断抗碳酸酐酶Ⅱ抗体出现的指标。

关键词: 自身抗体, 碳酸酐酶Ⅱ, 碳酸酐酶Ⅲ, 充血性心力衰竭

Abstract: Objective To determine serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibodies, the level of antioxidants and cytokines in patients with congestive heart failure (CHF) by established methods, and to evaluate primarily the clinical significance. Methods The serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibodies, erythrocyte sedimentation rate(ESR), the levels of 5 kinds of cytokines [like interleukin 2 (IL-2)] and 5 kinds of antioxidants [like superoxide dismutase (SOD)] from 58 patients with CHF in recent 3 months to 1 year were determined.Results The level of serum anti-carbonic anhydrase Ⅱ auto-antibody in CHF patients was significantly higher than that in control group (P<0.05). The levels of SOD, glutathione peroxidase (GPx), catalase (CAT) and total antioxidant capacity (TAC) in CHF patients were significantly lower than those in control group (P<0.05), while methanedicarboxylic aldehyde (MDA) level was significantly higher than that in control group(P<0.05). There was no statistical significance on the levels of interleukin (IL-6), tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) between CHF patients and control group (P>0.05), while IL-2 and interleukin 17(IL-17) levels were significantly higher than those in control group (P<0.05). Conclusions There may be autoimmune phenomenon in CHF patients. IL-2, IL-17 and antioxidant capacity may be used as the markers for the diagnosis of anti-carbonic anhydrase Ⅱ auto-antibody.

Key words: Auto-antibody, Carbonic anhydrase Ⅱ, Carbonic anhydrase Ⅲ, Congestive heart failure

中图分类号:

R446.62

刘辰庚, 夏鸣, 王金玲, 李蕾, 王培昌. 充血性心力衰竭患者血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体检测的意义[J]. 检验医学, 2014, 29(2): 97-99.

LIU Chengeng, XIA Ming, WANG Jinling, LI Lei, WANG Peichang. The significance of serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibody determination in patients with congestive heart failure[J]. , 2014, 29(2): 97-99.

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充血性心力衰竭患者血清抗碳酸酐酶Ⅱ和Ⅲ自身抗体检测的意义

The significance of serum anti-carbonic anhydrase Ⅱ and Ⅲ auto-antibody determination in patients with congestive heart failure

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