改良亚胺培南-EDTA纸片增效试验检测铜绿假单胞菌产金属酶表型

doi:10.3969/j.issn.1673-8640.2014.01.016

检验医学 ›› 2014, Vol. 29 ›› Issue (1): 65-68.DOI: 10.3969/j.issn.1673-8640.2014.01.016

• 技术研究与评价·论著 • 上一篇下一篇

改良亚胺培南-EDTA纸片增效试验检测铜绿假单胞菌产金属酶表型

严育忠, 范惠清, 郑文龙, 杨焕章, 陆燕春, 石毅

上海市浦东医院检验科, 上海 201300

收稿日期:2013-03-03 出版日期:2014-01-20 发布日期:2014-01-20
通讯作者: 石毅, 联系电话:021-58022995。
作者简介:严育忠, 男, 1977年生, 硕士, 主管技师, 主要从事细菌耐药机制研究。
基金资助:
上海市浦东医院“南箐奖”资助项目

The phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa by modified imipenem-EDTA disk potentiation test

YAN Yuzhong, FAN Huiqing, ZHENG Wenlong, YANG Huanzhang, LU Yanchun, SHI Yi.

Department of Clinical Laboratory, Pudong Hospital, Shanghai 201300, China

Received:2013-03-03 Online:2014-01-20 Published:2014-01-20

摘要/Abstract

摘要： 目的评估改良亚胺培南-乙二胺四乙酸(EDTA)纸片增效试验在检测铜绿假单胞菌(PA)产金属酶表型中的应用价值。方法收集95株耐碳青霉烯类药物PA, 采用琼脂稀释法测定所有菌株对亚胺培南和美罗培南的最低抑菌浓度(MIC)值, 聚合酶链反应(PCR)和DNA测序鉴定金属酶基因型, 亚胺培南-EDTA纸片增效试验常规法和改良法检测金属酶表型。结果 95株耐碳青霉烯类药物PA对亚胺培南和美罗培南的耐药率分别为100.0%和71.6%;常规法敏感性、特异性、阳性预测值和阴性预测值分别是100.0%、96.6%、72.7%和100.0%, 而改良法均为100.0%。结果改良亚胺培南-EDTA纸片增效试验可能是一种检测PA产金属酶表型的有效方法。

关键词: 金属酶, 铜绿假单胞菌, 纸片增效试验

Abstract: Objective To evaluate the modified imipenem-ethylene diamine tetraacetic acid (EDTA) disk potentiation test for the phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa(PA). Methods A total of 95 carbapenem-resistant PA were collected. The minimum inhibition concentrations(MIC) of imipenem and meropenem were determined by agar dilution method.Polymerase chain reaction (PCR) and DNA sequencing were used for the identification of metallo-beta-lactamase genotypes. The phenotypic detection of metallo-beta-lactamase was done by conventional and modified imipenem-EDTA disk potentiation test. Results The resistance rates of imipenem and meropenem were 100.0% and 71.6% among these 95 carbapenem-resistant PA. The sensitivity, specificity, positive predictive value and negative predictive value of conventional test were 100.0%, 96.6%, 72.7% and 100.0%, respectively. Those of modified test were all 100.0%. Results The modified imipenem-EDTA disk potentiation test could be an effective method for the phenotypic detection of metallo-beta-lactamase-producing PA.

Key words: Metallo-beta-lactamase, Pseudomonas aeruginosa, Disk potentiation test

中图分类号:

R446.5

严育忠, 范惠清, 郑文龙, 杨焕章, 陆燕春, 石毅. 改良亚胺培南-EDTA纸片增效试验检测铜绿假单胞菌产金属酶表型[J]. 检验医学, 2014, 29(1): 65-68.

YAN Yuzhong, FAN Huiqing, ZHENG Wenlong, YANG Huanzhang, LU Yanchun, SHI Yi.. The phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa by modified imipenem-EDTA disk potentiation test[J]. , 2014, 29(1): 65-68.

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改良亚胺培南-EDTA纸片增效试验检测铜绿假单胞菌产金属酶表型

The phenotypic detection of metallo-beta-lactamase-producing Pseudomonas aeruginosa by modified imipenem-EDTA disk potentiation test

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