环二鸟苷酸调控铜绿假单胞菌生物被膜研究进展

doi:10.3969/j.issn.1673-8640.2023.02.017

检验医学 ›› 2023, Vol. 38 ›› Issue (2): 186-189.DOI: 10.3969/j.issn.1673-8640.2023.02.017

环二鸟苷酸调控铜绿假单胞菌生物被膜研究进展

费冰, 刘莹, 任彦颖, 郭梦雨, 刘心伟, 刘冬梅, 李永伟()

河南中医药大学第二临床医学院郑州市病原微生物重点实验室，河南郑州 450002

收稿日期:2021-12-27 修回日期:2022-03-01 出版日期:2023-02-28 发布日期:2023-04-17
通讯作者: 李永伟，E-mail：lyw@hactcm.edu.cn。
作者简介:费冰，女，1998年生，学士，主要从事病原微生物耐药研究。
基金资助:
河南省自然科学基金面上项目(202300410263);河南中医药大学研究生科研创新类项目(2021KYCX028)

Research progress of cyclic diguanylate on the regulation of Pseudomonas aeruginosa biofilm

FEI Bing, LIU Ying, REN Yanying, GUO Mengyu, LIU Xinwei, LIU Dongmei, LI Yongwei()

The Second Clinical Medical College of Henan University of Traditional Chinese Medicine，Key Laboratory of Pathogenic Microorganisms of Zhengzhou，Zhengzhou 450002，Henan，China

Received:2021-12-27 Revised:2022-03-01 Online:2023-02-28 Published:2023-04-17

摘要/Abstract

摘要：

从浮游到生物被膜（BF）生命周期的转变能够促进细菌对非生物表面的黏附，逃避宿主免疫系统，增强其对常见抗菌药物的抗性，在导致人类病原菌感染率不断增加的同时，也大大增加了临床治疗的难度。第二信使环二鸟苷酸（c-di-GMP）对 BF的调控起重要作用。通过直接靶向c-di-GMP信号传导抑制抗菌药物抗性和BF形成意义重大。文章对c-di-GMP在铜绿假单胞菌BF调控中的作用进行综述。

关键词: 环二鸟苷酸, 铜绿假单胞菌, 生物被膜, 调控作用

Abstract:

The transition of bacteria from planktonic growth to biofilm（BF） life cycle can promote the adhesion of bacteria to non-biological surfaces，evade host immune system，and increase resistance to common antibiotics. The current BF-forming bacteria have an increasing probability of human infection and greatly increase the energy and time required for treatment and recovery. The second messenger cyclic diguanylate（c-di-GMP） plays a role in the regulation of BF. The research on the treatment of antibiotic-resistant and BF-forming bacteria by directly targeting c-di-GMP signaling has shown promise. Therefore，this review summarizes the effect of c-di-GMP on the regulation of Pseudomonas aeruginosa BF.

Key words: Cyclic diguanylate, Pseudomonas aeruginosa, Biofilm, Regulation

中图分类号:

R446.1

费冰, 刘莹, 任彦颖, 郭梦雨, 刘心伟, 刘冬梅, 李永伟. 环二鸟苷酸调控铜绿假单胞菌生物被膜研究进展[J]. 检验医学, 2023, 38(2): 186-189.

FEI Bing, LIU Ying, REN Yanying, GUO Mengyu, LIU Xinwei, LIU Dongmei, LI Yongwei. Research progress of cyclic diguanylate on the regulation of Pseudomonas aeruginosa biofilm[J]. Laboratory Medicine, 2023, 38(2): 186-189.

参考文献 36

[1]	SHARIATI A, AZIMI T, ARDEBILI A, et al. Insertional inactivation of oprD in carbapenem-resistant Pseudomonas aeruginosa strains isolated from burn patients in Tehran,Iran[J]. New Microbes New Infect, 2017, 21:75-80. DOI URL
[2]	GOMILA A, CARRATALÀ J, BADIA J M, et al. Preoperative oral antibiotic prophylaxis reduces Pseudomonas aeruginosa surgical site infections after elective colorectal surgery:a multicenter prospective cohort study[J]. BMC Infect Dis, 2018, 18(1):507. DOI
[3]	HASSETT D J, KOVALL R A, SCHURR M J, et al. The bactericidal tandem drug,AB569:how to eradicate antibiotic-resistant biofilm Pseudomonas aeruginosa in multiple disease settings including cystic fibrosis,burns/wounds and urinary tract infections[J]. Front Microbiol, 2021, 12:639362. DOI URL
[4]	GALE M J, MARITATO M S, CHEN Y L, et al. Pseudomonas aeruginosa causing inflammatory mass of the nasopharynx in an immunocompromised HIV infected patient:a mimic of malignancy[J]. IDCases, 2015, 2(2):40-43. DOI URL
[5]	全国细菌耐药监测网. 全国细菌耐药监测网2014—2019年细菌耐药性监测报告[J]. 中国感染控制杂志, 2021, 20(1):15-30.
[6]	BHARDWAJ S, BHATIA S, SINGH S, et al. Growing emergence of drug-resistant Pseudomonas aeruginosa and attenuation of its virulence using quorum sensing inhibitors:a critical review[J]. Iran J Basic Med Sci, 2021, 24(6):699-719.
[7]	WOLSKA K I, GRUDNIAK A M, RUDNICKA Z, et al. Genetic control of bacterial biofilms[J]. J Appl Genet, 2016, 57(2):225-238. DOI PMID
[8]	VALENTINI M, FILLOUX A. Biofilms and cyclic di-GMP(c-di-GMP)signaling:lessons from Pseudomonas aeruginosa and other bacteria[J]. J Biol Chem, 2016, 291(24):12547-12555. DOI URL
[9]	KARATAN E, WATNICK P. Signals,regulatory networks,and materials that build and break bacterial biofilms[J]. Microbiol Mol Biol Rev, 2009, 73(2):310-347. DOI URL
[10]	WEI Q, MA L Z. Biofilm matrix and its regulation in Pseudomonas aeruginosa[J]. Int J Mol Sci, 2013, 14(10):20983-21005. DOI URL
[11]	王帅涛, 高倩倩, 成娟丽, 等. 铜绿假单胞菌生物被膜组成及其受群体感应系统和c-di-GMP调控的研究进展[J]. 微生物学报, 2021, 61(5):1106-1122.
[12]	THI M T T, WIBOWO D, REHM B H A. Pseudomonas aeruginosa biofilms[J]. Int J Mol Sci, 2020, 21(22):8671. DOI URL
[13]	BELLINI D, CALY D L, MCCARTHY Y, et al. Crystal structure of an HD-GYP domain cyclic-di-GMP phosphodiesterase reveals an enzyme with a novel trinuclear catalytic iron centre[J]. Mol Microbiol, 2014, 91(1):26-38. DOI PMID
[14]	CHOU S H, GALPERIN M Y. Diversity of cyclic di-GMP-binding proteins and mechanisms[J]. J Bacteriol, 2016, 198(1):32-46. DOI URL
[15]	GALPERIN M Y. Diversity of structure and function of response regulator output domains[J]. Curr Opin Microbiol, 2010, 13(2):150-159. DOI PMID
[16]	KULASAKARA H, LEE V, BRENCIC A, et al. Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence[J]. Proc Natl Acad Sci U S A, 2006, 103(8):2839-2844. DOI URL
[17]	MERRITT J H, BROTHERS K M, KUCHMA S L, et al. SadC reciprocally influences biofilm formation and swarming motility via modulation of exopolysaccharide production and flagellar function[J]. J Bacteriol, 2007, 189(22):8154-8164. DOI PMID
[18]	KUCHMA S L, BROTHERS K M, MERRITT J H, et al. BifA,a cyclic-Di-GMP phosphodiesterase,inversely regulates biofilm formation and swarming motility by Pseudomonas aeruginosa PA14[J]. J Bacteriol, 2007, 189(22):8165-8178. DOI URL
[19]	HICKMAN J W, TIFREA D F, HARWOOD C S. A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels[J]. Proc Natl Acad Sci U S A, 2005, 102(40):14422-14427. DOI URL
[20]	KATHARIOS-LANWERMEYER S, WHITFIELD G B, HOWELL P L, et al. Pseudomonas aeruginosa uses c-di-GMP phosphodiesterases RmcA and MorA to regulate biofilm maintenance[J]. mBio, 2021, 12(1):e03384-20.
[21]	SULTAN M, ARYA R, KIM K K. Roles of two-component systems in Pseudomonas aeruginosa virulence[J]. Int J Mol Sci, 2021, 22(22):12152. DOI URL
[22]	MERIGHI M, LEE V T, HYODO M, et al. The second messenger bis-(3'-5')-cyclic-GMP and its PilZ domain-containing receptor Alg44 are required for alginate biosynthesis in Pseudomonas aeruginosa[J]. Mol Microbiol, 2007, 65(4):876-895. DOI URL
[23]	MORGAN J L, MCNAMARA J T, ZIMMER J. Mechanism of activation of bacterial cellulose synthase by cyclic di-GMP[J]. Nat Struct Mol Biol, 2014, 21(5):489-496. DOI PMID
[24]	WHITNEY J C, WHITFIELD G B, MARMONT L S, et al. Dimeric c-di-GMP is required for post-translational regulation of alginate production in Pseudomonas aeruginosa[J]. J Biol Chem, 2015, 290(20):12451-12462. DOI URL
[25]	MORADALI M F, GHODS S, REHM B H A. Activation mechanism and cellular localization of membrane-anchored alginate polymerase in Pseudomonas aeruginosa[J]. Appl Environ Microbiol, 2017, 83(9):e03499-16.
[26]	WHITFIELD G B, HOWELL P L. The matrix revisited:opening night for the Pel polysaccharide across eubacterial kingdoms[J]. Microbiol Insights, 2021, 14:1178636120988588.
[27]	LEE V T, MATEWISH J M, KESSLER J L, et al. A cyclic-di-GMP receptor required for bacterial exopolysaccharide production[J]. Mol Microbiol, 2007, 65(6):1474-1484. PMID
[28]	WHITNEY J C, COLVIN K M, MARMONT L S, et al. Structure of the cytoplasmic region of PelD,a degenerate diguanylate cyclase receptor that regulates exopolysaccharide production in Pseudomonas aeruginosa[J]. J Biol Chem, 2012, 287(28):23582-23593. DOI URL
[29]	WHITFIELD G B, MARMONT L S, OSTASZEWSKI A, et al. Pel polysaccharide biosynthesis requires an inner membrane complex comprised of PelD,PelE,PelF,and PelG[J]. J Bacteriol, 2020, 202(8):e00684-19.
[30]	POULIN M B, KUPERMAN L L. Regulation of biofilm exopolysaccharide production by cyclic di-guanosine monophosphate[J]. Front Microbiol, 2021, 12:730980. DOI URL
[31]	CHAMBERS J R, CHERNY K E, SAUER K. Susceptibility of Pseudomonas aeruginosa dispersed cells to antimicrobial agents is dependent on the dispersion cue and class of the antimicrobial agent used[J]. Antimicrob Agents Chemother, 2017, 61(12):e00846-17.
[32]	ANDERSEN J B, KRAGH K N, HULTQVIST L D, et al. Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms[J]. Antimicrob Agents Chemother, 2021, 65(4):e02431-20.
[33]	ZHANG Y, SASS A, VAN ACKER H, et al. Coumarin reduces virulence and biofilm formation in Pseudomonas aeruginosa by affecting quorum sensing,type Ⅲ secretion and c-di-GMP levels[J]. Front Microbiol, 2018, 9:1952. DOI URL
[34]	KIM S K, NGO H X, DENNIS E K, et al. Inhibition of Pseudomonas aeruginosa alginate synthesis by ebselen oxide and its analogues[J]. ACS Infect Dis, 2021, 7(6):1713-1726. DOI URL
[35]	HOU L, DEBRU A, CHEN Q, et al. AmrZ regulates swarming motility through cyclic di-GMP-dependent motility inhibition and controlling Pel polysaccharide production in Pseudomonas aeruginosa PA14[J]. Front Microbiol, 2019, 10:1847. DOI URL
[36]	DE SMET J, WAGEMANS J, HENDRIX H, et al. Bacteriophage-mediated interference of the c-di-GMP signalling pathway in Pseudomonas aeruginosa[J]. Microb Biotechnol, 2021, 14(3):967-978. DOI URL

环二鸟苷酸调控铜绿假单胞菌生物被膜研究进展

Research progress of cyclic diguanylate on the regulation of Pseudomonas aeruginosa biofilm

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献 36

相关文章 15

编辑推荐

Metrics

[1]	陈庆, 徐蓉, 孙景勇. 上海地区支气管扩张症患者铜绿假单胞菌耐药机制和分子流行病学特征[J]. 检验医学, 2023, 38(11): 1020-1025.
[2]	李婵婵, 王茹, 罗文英. 铜绿假单胞菌转录因子PsrA全局调控研究进展[J]. 检验医学, 2020, 35(8): 832-836.
[3]	钟峰, 冯艺, 张晶晶, 许岩, 方咏梅, 陶秀彬, 吴竞. 替加环素联合亚胺培南对黏液型铜绿假单胞菌的体外抗菌活性及生物膜抑制作用分析[J]. 检验医学, 2020, 35(6): 519-523.
[4]	毛磊, 王炜, 苏建荣. 北京市某医院354株铜绿假单胞菌分布及耐药情况分析[J]. 检验医学, 2019, 34(6): 502-505.
[5]	范雪梅. 亚抑菌浓度阿奇霉素与妥布霉素联合应用对铜绿假单胞菌疗效的分析[J]. 检验医学, 2017, 32(10): 879-882.
[6]	黄秋兰, 范德平, 高守霞, 刘红绸, 薛娜丽, 倪语星. Ⅰ类整合子对铜绿假单胞菌亚胺培南耐药诊断价值的Meta分析[J]. 检验医学, 2016, 31(6): 491-498.
[7]	徐伟红, 徐斌, 姚怡婷, 黄明敏, 张骏, 赵虎. 铜绿假单胞菌产AmpC β-内酰胺酶及外膜孔蛋白OprD₂基因缺失分析[J]. 检验医学, 2016, 31(4): 309-313.
[8]	舒文, 钱颖, 刘庆中. 上海市某教学医院2010至2014年铜绿假单胞菌耐药性变迁分析[J]. 检验医学, 2016, 31(12): 1055-1060.
[9]	彭敬红, 侯彦强, 谢多双, 刘军, 郑蓉. 铜绿假单胞菌16S rRNA甲基化酶基因和氨基糖苷修饰酶基因的研究[J]. 检验医学, 2015, 30(6): 613-616.
[10]	解范迪, 牛术孟, 施锦杰, 张雯雁. 产志贺毒素大肠埃希菌生物被膜形成能力与毒力基因表达的关系[J]. 检验医学, 2015, 30(6): 617-621.
[11]	张勇，刘爱胜，文艳. 75株碳青霉烯类耐药铜绿假单胞菌ERIC-PCR菌种分型及其主要耐药机制研究[J]. 检验医学, 2014, 29(7): 755-759.
[12]	张国栋,王莹,朱红胜. 临床分离的美罗培南和环丙沙星共同耐药的铜绿假单胞菌耐药机制的研究[J]. 检验医学, 2014, 29(6): 646-650.
[13]	李利燕, 李洪春, 马萍. 穿心莲内酯在铜绿假单胞菌诱导的大鼠肺部感染模型中的抗炎作用及其作用机制[J]. 检验医学, 2014, 29(5): 535-539.
[14]	曾章锐，王卫萍，黄梅，邵海枫. 临床分离的铜绿假单胞菌对头孢吡肟敏感性低于头孢他啶的机制研究[J]. 检验医学, 2014, 29(11): 1178-1183.
[15]	严育忠, 范惠清, 郑文龙, 杨焕章, 陆燕春, 石毅. 改良亚胺培南-EDTA纸片增效试验检测铜绿假单胞菌产金属酶表型[J]. 检验医学, 2014, 29(1): 65-68.