Abstract:

Objective To investigate the role of peripheral blood long non-coding RNA（lncRNA）HOTTIP and lncRNA HULC expression in multiple myeloma（MM）monitoring and prognosis assessment. Methods Totally，67 MM patients from Yancheng Third People's Hospital（the Affiliated Hospital of Jiangsu Vocational College of Medicine）from January 2021 to January 2023 were enrolled as MM group. All the patients were classified into stage Ⅰ group（20 cases），stage Ⅱ group（31 cases）and stage Ⅲ group（16 cases）according to the International Staging System（ISS）. All the patients were followed up for 2 years，and they were classified into death group（22 cases）and survival group（45 cases）based on prognosis. Totally，67 healthy subjects were enrolled as healthy control group. The clinical data were collected，and the relative expression levels of serum lncRNA HOTTIP and lncRNA HULC were determined. Receiver operating characteristic（ROC）curve was used to evaluate the efficacy of each indicator in determining the ISS stage and death of MM patients. Cox regression analysis was used to assess the influencing factors of death in MM patients. The survival status of MM patients was analyzed using Kaplan-Meier survival curve. Results The relative expression levels of serum lncRNA HOTTIP and lncRNA HULC in MM group were higher than those in healthy control group（P<0.001）. The relative expression levels of serum lncRNA HOTTIP and lncRNA HULC in stage Ⅰ group，stage Ⅱ group and stage Ⅲ group were increased successively（P<0.001）. The areas under curves（AUC）of single and combined determinations of serum lncRNA HOTTIP and lncRNA HULC in determining the ISS stage of MM patients as stage Ⅲ were 0.830，0.852 and 0.910，respectively. The serum levels of lncRNA HOTTIP and lncRNA HULC in death group were higher than those in survival group（P<0.001）. The ISS stage，lncRNA HOTTIP and lncRNA HULC were all risk factors for the death of MM patients（P<0.001）. The AUC of single and combined determinations of serum lncRNA HOTTIP and lncRNA HULC for predicting the death of MM patients were 0.850，0.873 and 0.937，respectively. The 2-year survival rate was lower in high-expression groups of lncRNA HOTTIP and lncRNA HULC compared to their low-expression groups（P<0.01）. Conclusions The expressions of serum lncRNA HOTTIP and lncRNA HULC are related to the severity and mortality of MM，which may serve as molecular markers for the assessment of MM monitoring and prognosis.

Key words: Long non-coding RNA HOTTIP, Long non-coding RNA HULC, Multiple myeloma