TSA induced cell cycle apoptosis in nasopharyngeal carcinoma CNE2 cells and its mechanisms

Abstract

Abstract: Objective　To investigate the influence of trichostatin A (TSA) on cell apoptosis in nasopharyngeal carcinoma CNE2 cells and its mechanism. Methods　CNE2 cells were incubated with TSA at different concentrations (300, 400 and 500 nmol/L). The effects of TSA on cell apoptosis were detected by flow cytometry. The expression of cyclin-dependent kinase inhibitor 1A (P21) was analyzed by reverse transcription-polymerase chain reaction (RT-PCR). Results　The apoptosis rates with different concentrations of TSA (300, 400 and 500 nmol/L) were 12.52%±1.57%，19.69%±3.00% and 19.63%±2.68% after 24 h treatment. The apoptosis rates were 24.50%±4.45%，36.24%±3.92% and 34.82%±6.45% after 48 h treatment. There were statistical significance compared with those of controls (0 nmol/L TSA, P<0.05). The expression level of p21 increased after TSA treatment (P<0.01). Conclusions　TSA markedly induces CNE2 cell apoptosis. Its mechanism would be related with the abnormal expression of p21 gene.

Key words: Trichostatin A, CNE2 cell line, Apoptosis, p21 gene

YANG Huimin;HUANG Lin;QIN Mengbin;ZHONG Yueyuan;LIU Xiaochun;YANG Xiaoli. TSA induced cell cycle apoptosis in nasopharyngeal carcinoma CNE2 cells and its mechanisms[J]. , 2012, 27(5): 371-373.

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