Influence of baicalein on in vitro anti-tumor activity of docetaxel for prostate cancer

doi:10.3969/j.issn.1673-8640.2018.06.019

Abstract

Abstract:

Objective To investigate the influence and mechanism of baicalein on in vitro anti-tumor activity of docetaxel for prostate cancer. Methods According to different processing modes,human prostate cancer LNCaP cells were classified into control group（empty plasmid transfection）,baicalein group（10 mmol/L baicalein）,docetaxel group（1 nmol/L docetaxel）,docetaxel+baicalein group（1 nmol/L docetaxel and 10 mmol/L baicalein） and docetaxel+baicalein+murine double minute 2（MDM2） plasmid group （1 nmol/L docetaxel and 10 mmol/L baicalein after MDM2 plasmid transfection）. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） assay was performed to determine the activity of LNCaP cells. Flow cytometry was used to determine the apoptosis of LNCaP cells. Western blotting analysis was performed to determine the expression levels of MDM2,protein 53（p53）,phorbol-12-myriistate-13-acetate-induced protein 1（PMAIP1,also known as Noxa） and p53 upregulated modulator of apoptosis（Puma） and the activation levels of cysteine-containing aspartate-specific protease（Caspase）-9 and Caspase-3 in LNCaP cells. Results The activity inhibitory rate and apoptotic rate of LNCaP cells in docetaxel+baicalein group were higher than those in docetaxel+baicalein+MDM2 plasmid group,docetaxel group,baicalein group and control group（P<0.05）. The levels of MDM2 mRNA and MDM2 protein in LNCaP cells of baicalein group and docetaxel+baicalein group were lower than those in docetaxel+baicalein+MDM2 plasmid group,docetaxel group and control group（P<0.05）. The p53,Puma and Noxa expression levels,the releasing levels of cytochrome c and the activation levels of Caspase-9 and Caspase-3 in docetaxel+baicalein group were higher than those in docetaxel+baicalein+MDM2 plasmid group,docetaxel group,baicalein group and control group（P<0.05）,while relatively mitochondrial membrane potential was lower than those in the other groups（P<0.05）. Conclusions Baicalein increases the in vitro anti-tumor activity of docetaxel for prostate cancer through MDM2/p53.

Key words: Baicalein, Docetaxel, Prostate cancer, Murine double minute 2, Protein 53, Apoptosis

CLC Number:

R446.1

YING Xiao, WANG Yuying, WANG Zaihong, WANG Zhenhua. Influence of baicalein on in vitro anti-tumor activity of docetaxel for prostate cancer[J]. Laboratory Medicine, 2018, 33(6): 556-562.

Figures/Tables 7

References 17

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组别	细胞活力抑制率	凋亡率
对照组	0	1.5±0.2
黄芩素组	8.8±0.7	4.5±0.5
多西他赛组	19.8±1.6^*#△	10.6±0.8^*#△
多西他赛+黄芩素组	68.6±6.3^*#	38.1±3.3^*#
多西他赛+黄芩素+ MDM2质粒组	27.9±2.4^*#△	14.6±1.3^*#△

组别	细胞活力抑制率	凋亡率
对照组	0	1.5±0.2
黄芩素组	8.8±0.7	4.5±0.5
多西他赛组	19.8±1.6^*#△	10.6±0.8^*#△
多西他赛+黄芩素组	68.6±6.3^*#	38.1±3.3^*#
多西他赛+黄芩素+ MDM2质粒组	27.9±2.4^*#△	14.6±1.3^*#△