Abstract: Objective　To detect the serum anti-nuclear antibodies (ANA) and investigate its clinical significance in patients with hepatitis B virus (HBV) infection.   Methods　A total of 120 patients with HBV infection and 82 healthy subjects were enrolled， and indirect immuno-fluorescent assay (IIF) was used to detect the serum ANA. Results　The positive rate of ANA in 120 patients with HBV infection (30.0%) was higher than that of healthy subjects (4.2%). ANA in patients with HBV infection was mainly depended on low titer(1∶100, 58.3%). The ANA granular fluorescence (41.7%) was highest in the patients with HBV infection. Conclusions　HBV infection can induce to produce a variety of low titer ANA， and may play a certain role in the diagnosis， differential diagnosis and treatment. The pathogenesis of HBV may have some relationship with autoimmune.

Key words: Hepatitis B virus infection, Anti-nuclear antibody, Indirect immuno-fluorescent assay