Laboratory Medicine ›› 2019, Vol. 34 ›› Issue (6): 506-512.DOI: 10.3969/j.issn.1673-8640.2019.06.006

• Orginal Article • Previous Articles     Next Articles

Distribution and drug resistance of pathogens isolated from blood specimens from 2013 to 2018

YAO Rongfeng1, FANG Hui1, XU Guoxiang2, WU Yazhou1, REN Shengjie1, LI Zhi1   

  1. 1. Department of Clinical Laboratory,Yangpu Hospital of Tongji University,Shanghai 200090,China
    2. Sunqiao Community Health Service Center,Shanghai 201210,China
  • Received:2019-02-14 Online:2019-06-30 Published:2019-07-04

Abstract:

Objective To analyze the distribution and drug resistance of pathogens isolated from blood specimens from 2013 to 2018 in Yangpu Hospital of Tongji University,and to provide a reference for clinical anti-infection treatment. Methods The bacteria were identified,and drug susceptibility test was performed by automatic identification system. The distribution and drug resistance characteristics were analyzed. Results A total of 1 803 isolates of pathogens were isolated from 14 085 cases of blood culturing from 2013 to 2018. The positive rate was 12.8%. Among them,Gram-negative bacilli,Gram-positive cocci and fungi accounted for 51.1%,46.4% and 2.4%,respectively. The top 5 pathogens were coagulase-negative Staphylococcus,Escherichia coli,Staphylococcus aureus,Klebsiella pneumoniae and Enterococcus faecalis. Intensive care unit(ICU)(19.3%) had the highest isolation rate of pathogens from blood culturing. The isolation rate of Escherichia coli and Klebsiella pneumoniae from department of surgery were 32.8% and 14.2%,respectively,which were higher than those in internal medicine department(26.8%% and 6.9%) and ICU(16.1% and 6.3%)(P<0.05). The isolation rate of Escherichia coli from female patients were 33.0%,which was higher than that from male patients(20.7%)(P<0.05). Imipenem,meropenem,amikacin,cefoperazone/sulbactam,piperacillin/tazobactam maintained good antimicrobial activity against Escherichia coli,Klebsiella pneumoniae,Pseudomonas aeruginosa and Serratia marcescens,and the drug resistance rates were all <15%. The drug resistance rates of Acinetobacter baumannii to most antibiotics were >40%. Staphylococcus was found not to be resistant to linezolid,tigecycline and vancomycin. The drug resistance rate of Enterococcus faecalis to linezolid was 1.6%. The drug resistance rate of Enterococcus faecium to vancomycin was 7.4%. Conclusions The kinds of pathogens isolated from blood culturing are complex. Clinicians should standardize the use of antibiotics according to drug susceptibility test results of positive pathogens from blood culturing.

Key words: Blood culturing, Pathogen, Drug resistance

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