MALDI-TOF MS in the determination of drug resistance

doi:10.3969/j.issn.1673-8640.2017.01.014

Abstract

Abstract:

The application of matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF MS） has brought a revolutionary change for microbial identification program in clinical microbiological laboratories,for its fast,high-throughput,accuracy and cost-effectiveness. Furthermore,MALDI-TOF MS provides a new way for drug resistance determination. It is time-consuming for commonly-used methods of routine drug resistance determination,such as Kirby-Bauer,dilution and E-test. These methods can not meet the clinical demand for quick selection of sensitive drugs. So,the application of MALDI-TOF MS for the determination of drug resistance has research value and prospect. Some experiments and clinical application of MALDI-TOF MS for determining drug resistance have been reported. This review introduces the types of pathogens,determination methods and the efficiency of MALDI-TOF MS for determining drug resistance in recent years.

Key words: Matrix-assisted laser desorption ionization time of flight mass spectrometry, Bacteria, Drug resistance

CLC Number:

R446.5

YANG Feng, CHEN Fei, ZHAO Hu. MALDI-TOF MS in the determination of drug resistance[J]. Laboratory Medicine, 2017, 32(1): 57-63.

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