Antibacterial activity of 4 Chinese herbal medicines alone and combined with western medicines for extensively drug resistant Acinetobacter baumannii

doi:10.3969/j.issn.1673-8640.2023.05.007

Abstract

Abstract:

Objective To analyze the antibacterial effects of Radix isatidis，Forsythia，Scutellaria baicalensis and Dandelion and combined with cefoperazone-sulbactam（SCF） for extensively drug-resistant Acinetobacter baumannii（XDR-AB）. Methods Totally，30 isolates of XDR-AB were collected from clinical specimens from September 2018 to October 2019 in the Third Affiliated Hospital of Beijing University of Chinese Medicine. The minimum inhibitory concentrations（MIC） of Radix isatidis，Forsythia，Scutellaria baicalensis and Dandelion alone and combined with SCF to 30 isolates of XDR-AB were determined by broth microdilution method. Fractional inhibitory concentrations（FIC） were calculated. The changes of K⁺ and alkaline phosphatase（ALP）levels of 4 Chinese herbal medicines were compared. Results The 4 Chinese herbal medicines have certain antibacterial effects. The MIC of Forsythia and Scutellaria baicalensis were low. The effect of Radix isatidis combined with SCF was good，and synergistic and additive effects were predominant. The effects of Forsythia and Scutellaria baicalensis combined with SCF were mainly antagonism（FIC index >2）. The K⁺ levels of the 4 groups were not statistically significant（P>0.05）. The ALP levels in Radix isatidis and Dandelion groups were not statistically significant（P<0.05），and those in Forsythia and Scutellaria baicalensis groups were not statistically significant（P>0.05）. Conclusions The antibacterial effects of Forsythia and Scutellaria baicalensis are good，the combined antibacterial effect of Radix isatidis and SCF is opitmal.

Key words: Chinese herbal medicine, Radix isatidis, Forsythia, Scutellaria baicalensis, Dandelion, Extensively drug-resistant Acinetobacter baumannii, Combined medication, Antibacterial effect

CLC Number:

R285.6

MA Dongmei, JIANG Dongkui, XIANG Xiaobo, LI Shiliang. Antibacterial activity of 4 Chinese herbal medicines alone and combined with western medicines for extensively drug resistant Acinetobacter baumannii[J]. Laboratory Medicine, 2023, 38(5): 441-445.

Figures/Tables 8

References 17

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药物名称	单位	单用			联用
药物名称	单位	MIC范围	MIC₅₀	MIC₉₀	MIC范围	MIC₅₀	MIC₉₀
板蓝根	mg/mL	31.2~125.0	62.5	62.5	15.6~62.5	31.2	62.5
SCF	μg/mL	64.0~256.0	64.0	128.0	16.0~128.0	32.0	64.0

药物名称	单位	单用			联用
药物名称	单位	MIC范围	MIC₅₀	MIC₉₀	MIC范围	MIC₅₀	MIC₉₀
板蓝根	mg/mL	31.2~125.0	62.5	62.5	15.6~62.5	31.2	62.5
SCF	μg/mL	64.0~256.0	64.0	128.0	16.0~128.0	32.0	64.0

药物名称	单位	单用			联用
药物名称	单位	MIC范围	MIC₅₀	MIC₉₀	MIC范围	MIC₅₀	MIC₉₀
连翘	mg/mL	2.0~31.2	3.9	7.8	2.0~31.2	7.8	7.8
SCF	μg/mL	64.0~256.0	64.0	128.0	32.0~256.0	128.0	256.0

药物名称	单位	单用			联用
药物名称	单位	MIC范围	MIC₅₀	MIC₉₀	MIC范围	MIC₅₀	MIC₉₀
连翘	mg/mL	2.0~31.2	3.9	7.8	2.0~31.2	7.8	7.8
SCF	μg/mL	64.0~256.0	64.0	128.0	32.0~256.0	128.0	256.0

药物名称	单位	单用			联用
药物名称	单位	MIC范围	MIC₅₀	MIC₉₀	MIC范围	MIC₅₀	MIC₉₀
黄芩	mg/mL	1.0~7.8	3.9	3.9	2.0~15.6	7.8	7.8
SCF	μg/mL	64.0~256.0	64.0	128.0	32.0~256.0	128.0	256.0