Improving laboratory diagnosis ability of infectious diseases in children using multiple assays

doi:10.3969/j.issn.1673-8640.2016.03.001

Abstract

Abstract:

Infectious disease is a major problem for children' s health worldwide. Early and accurate identification and diagnosis of pathogens play a very important role for the prevention and control of infectious diseases,especially for the control of antibiotics and anti-viral medicine abuse. In order to improve the laboratory ability for the diagnosis of infectious diseases in children,a variety of clinical and on-site diagnostic systems should be developed for the rapid determination of pathogens or host biomarkers by utilizing multiple techniques and assays,and the molecular epidemiological characteristics of pathogen genotype and its possible relationship with pathogenesis should be deeply investigated.

Key words: Child, Infectious diseases, Diagnosis, Isothermal amplification, Biomarkers

CLC Number:

R446.1

XU Jin.. Improving laboratory diagnosis ability of infectious diseases in children using multiple assays[J]. Laboratory Medicine, 2016, 31(3): 155-158.

References 12

1	YAN L,ZHOU J,ZHENG Y,et al.Isothermal amplified detection of DNA and RNA[J]. Mol Biosyst,2014,10(5):970-1003.
2	SIDOTI F,BERGALLO M,COSTA C,et al.Alternative molecular tests for virological diagnosis[J]. Mol Biotechnol,2013,53(3):352-362.
3	CHANG CC,CHEN CC,WEI SC,et al.Diagnostic devices for isothermal nucleic acid amplification[J]. Sensors(Basel),2012,12(6):8319-8337.
4	KAPLAN JM,WONG HR.Biomarker discovery and development in pediatric critical care medicine[J]. Pediatr Crit Care Med,2011,12(2):165-173.
5	SCHUETZ P,CHRIST-CRAIN M,MÜLLER B. Procalcitonin and other biomarkers to improve assessment and antibiotic stewardship in infections-hope for hype?[J]. Swiss Med Wkly,2009,139(23-24):318-326.
6	DUPUY AM,PHILIPPART F,PÉAN Y,et al. Role of biomarkers in the management of antibiotic therapy: an expert panel review:Ⅰ-currently available biomarkers for clinical use in acute infections[J]. Ann Intensive Care,2013,3(1):22.
7	STARKE JR,Committee on Infectious Diseases. Interferon-γ release assays for diagnosis of tuberculosis infection and disease in children[J]. Pediatrics,2014,134(6):e1763-e1773.
8	LEUNG CC,YAM WC,HO PL,et al.T-Spot.TB outperforms tuberculin skin test in predicting development of active tuberculosis among household contacts[J]. Respirology,2015,20(3):496-503.
9	JANG JY,PARK IW,CHOI BW,et al.The usefulness of the tuberculosis skin test and the interferon-gamma release assay in the diagnosis of latent tuberculosis infection in South Korea[J]. Osong Public Health Res Perspect,2014,5(Suppl):S18-S23.
10	屈园园,普雄明. 水痘带状疱疹病毒基因亚型的研究进展[J]. 医学综述,2014,22(4):601-604.
11	PARADOWSKA E,JABLONSKA A,STUDZINSKA M,et al.Distribution of cytomegalovirus gN variants and associated clinical sequelae in infants[J]. J Clin Virol,2013,58(1):271-275.
12	PATI SK,PINNINTI S,NOVAK Z,et al.Genotypic diversity and mixed infection in newborn disease and hearing loss in congenital cytomegalovirus infection[J]. Pediatr Infect Dis J,2013,32(10):1050-1054.

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