Transcription factor Flo8 G723R and T751D mutations enhancing virulence in Candida albicans

doi:10.3969/j.issn.1673-8640.2016.011.014

Abstract

Abstract:

Objective To study the relationship between Candida albicans transcription factor Flo8 G723R and T751D mutations and their virulence. Methods The lethality rate,renal fungal burden of infected mice and the expression of genes for virulence factors in Candida albicans Flo8 wild-type SN152,Flo8 complementation strain Flo8^SN152,Flo8 mutation strains Flo8^G723R and Flo8^T751D were compared. The relationship between Flo8 mutations and Candida albicans virulence was analyzed. Results Compared to SN152 and Flo8^SN152,Flo8 mutation strains Flo8^G723R and Flo8^T751D produced a higher lethality rate and more renal fungal burden of infected mice and the expression of genes for virulence factors,and increased at different degrees. Conclusions Transcription factor Flo8 G723R and T751D mutations increase the virulence and the expression of genes for partial virulence factors in Candida albicans.

Key words: G723R, T751D, Virulence, Transcription factor Flo8, Candida albicans

CLC Number:

R519.5

LI Wenjing, SHEN Yun, LIU Jinyan, SHI Ce, WANG Ying, ZHAO Yue, XIANG Mingjie. Transcription factor Flo8 G723R and T751D mutations enhancing virulence in Candida albicans[J]. Laboratory Medicine, 2016, 31(11): 987-992.

Figures/Tables 5

References 23

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菌株	基因型
SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4
Flo8^SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8-PAP
Flo8^G723R	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8G723R-PAP
Flo8^T751D	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8T751D-PAP

菌株	基因型
SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4
Flo8^SN152	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8-PAP
Flo8^G723R	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8G723R-PAP
Flo8^T751D	ura3::imm434::URA3/ura3::imm434iro1::IRO1/ iro1::imm434his1::hisG/his1::hisGleu2/leu2arg4/ arg4 flo8::CmLEU2/ flo8::CdHIS1 ADE2/ade2:: ADH1p-FLO8T751D-PAP

引物	序列（5'~3'）
ALS1-F	CATTTTCCCGTCCTCATT
ALS1-R	CATTGCTATCTGGATTTGTG
ALS5-F	AGTTATGGGGATGTTGCT
ALS5-R	CTGGTTATTATCTGAGGG
HWP1-F	AGGTAGACGGTCAAGGTG
HWP1-R	TTGTGGCTGTTGTGGATA
INT1-F	CATTTTAGCCAACTCGTC
INT1-R	GAATCAGTTTCGTGCTCT
MNT1-F	GTCCGTGAACAAATGAGA
MNT1-R	GGTAAGGAAATAGTGAATGC
SAP2-F	CTACTCCAACAACAACCA
SAP2-R	CATCAGGAACCCATAAATC
PLB1-F	TATCTCAAGGTTTGTGGG
PLB1-R	ATAAGCATCACCTTCGTC
18S RNA-F	TCTTGTACGGCACATATCTCGT
18S RNA-R	GTTTCGAGAGGACCTTCAGTC

引物	序列（5'~3'）
ALS1-F	CATTTTCCCGTCCTCATT
ALS1-R	CATTGCTATCTGGATTTGTG
ALS5-F	AGTTATGGGGATGTTGCT
ALS5-R	CTGGTTATTATCTGAGGG
HWP1-F	AGGTAGACGGTCAAGGTG
HWP1-R	TTGTGGCTGTTGTGGATA
INT1-F	CATTTTAGCCAACTCGTC
INT1-R	GAATCAGTTTCGTGCTCT
MNT1-F	GTCCGTGAACAAATGAGA
MNT1-R	GGTAAGGAAATAGTGAATGC
SAP2-F	CTACTCCAACAACAACCA
SAP2-R	CATCAGGAACCCATAAATC
PLB1-F	TATCTCAAGGTTTGTGGG
PLB1-R	ATAAGCATCACCTTCGTC
18S RNA-F	TCTTGTACGGCACATATCTCGT
18S RNA-R	GTTTCGAGAGGACCTTCAGTC

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