Analysis on the NA gene variation of influenza A (H1N1) virus from Guangzhou in 2010

doi:10.3969/j.issn.1673-8640.2014.01.010

Abstract

Abstract: Objective To compare the variations of neuraminidase (NA) gene of influenza A (H1N1) virus from Guangzhou in 2010 and NA gene of influenza A(H1N1) virus from Chinese mainland in 2009, and to provide the reference for the surveillance and prevention of influenza A (H1N1) virus. Methods Specimens were collected from patients with febrile respiratory tract symptoms from Guangzhou in 2010. The specimens were determined with influenza A (H1N1) virus specificity primers by polymerase chain reaction (PCR). The fragment of NA gene was amplified. After sequencing, the Results were compared with influenza A (H1N1) virus in 2009.The drug resistance sites and glycosylation sites were analyzed by biometric software. Results A total of 1 194 specimens were collected, and 327 influenza A virus strains were detected, including 6 strains were identified with influenza A (H1N1) virus. Compared with influenza A (H1N1) virus in 2009, there were 16 amino acid sites with variation. There were 3 amino acid sites relating with NA activity. However, the amino acid site 222 located in the NA activity sites. Results NA gene of the 6 strains of influenza A (H1N1) virus are amplified and sequenced successfully, and H275Y drug resistant site is not found. The amino acid site 222, 228 and 425 locating in NA activity sites are found with mutations in 3 strains. Therefore, the monitoring should be strengthened.

Key words: Influenza A( H1N1) virus, Neuraminidase, Drug resistance site

CLC Number:

R373.1

XU Shasha, CHANG Yanmin, XU Lin, FENG Fashen, HE Xia, WANG Zhu, ZHANG Dingmei, CAO Kaiyuan. Analysis on the NA gene variation of influenza A (H1N1) virus from Guangzhou in 2010[J]. , 2014, 29(1): 42-48.

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