Abstract: Abstract：Objective To establish the reference value of superoxide dismutase(SOD) measured by pyrogallol autoxidation method in healthy adults. Methods 1640 clinical serum samples for SOD were detected by pyrogallol autoxidation method in healthy adults，and the results were statisticed according to sex and age. Results The distribution of serum SOD was normal in health adults，and the levels were negatively correlated with ages（r=-0.541,P<0.01）.There was a significant difference in the levels of SOD between males and females（P<0.01）.The reference range in males: 147.6～218.4 U/mL in ≤40-year-old group，131.4～207.8 U/mL in 40 to 60-year-old group, 98.1～190.9 U/mL in ＞60-year-old group.And in females: 142.0～205.2 U/mL in ≤40-year-old group，126.4～194.4U/mL in 40 to 60-year-old group, 95.5～187.5 U/mL in ＞60-year-old group. Conclusions The reference range of serum SOD are inconsistent in different healthy people，it is essential to establish the rational reference value by respective detection system and detection method in different clinical laboratory.

Key words: Superoxide dismutase, Pyrogallol, Reference range