Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai

doi:10.3969/j.issn.1673-8640.2014.06.010

Abstract

Abstract: Objective To establish the reference ranges of peripheral blood T lymphocyte subsets among 333 healthy adults in Shanghai, to analyze and discuss the change characteristics through the establishment of 95% confidence interval, and to provide the reference for immune status analysis and clinical diagnosis and treatment. Methods Multiparameter three-color immunofluorescence was used to stain the peripheral blood T lymphocytes, and BD FACSCalibur flow cytometry was used to acquire and analyze the percentages and absolute counts of T lymphocyte subsets. Results Among females, the CD3⁺ percentage, CD4⁺/CD8⁺ ratio, CD3⁺ CD4⁺ percentage and CD4⁺CD45RA⁺ percentage (72.6%±8.1%), 1.57(0.74-4.70), (44.8%±8.0%) and (1.71%±6.3%) were respectively higher than those among males (70.9%±8.5%), 1.43(0.51-4.12), (40.6%±9.3%) and (15.1%±6.8%). The lymphocyte absolute count and CD8⁺CD28⁺ absolute count in males (2 069.8±474.1) cells/μL and (237.5±95.7) cells/μL were higher than those in females (1 932.8±469.7) cells/μL and (218.1±111.1) cells/μL. The percentage of T lymphocyte subsets increased with the increase of age.There were statistical significances in >50 year-old group with the other groups. The absolute count of 333 healthy adults for peripheral blood T lymphocyte was (1 069.1-2 935.6) cells/μL. The percentages of T lymphocyte subsets were: CD3⁺ 55.3%-87.6%, CD3⁺CD4⁺ 25.2%-57.6%, CD3⁺CD8⁺ 15.0%-42.1%, CD4⁺CD25⁺ 2.5%-10.5%, CD4⁺CD45RA⁺ 5.0%-27.2 % and CD8⁺CD28⁺ 4%-19.3%, and CD4⁺/CD8⁺ ratio was 0.7-2.9. Conclusions There are differences for the percentage distribution of T lymphocyte subsets among healthy adults between sex and age. Since these differences can be ignored, the reference ranges of peripheral blood T lymphocyte subsets among healthy adults could be established in Shanghai.

Key words: Flow cytometry, Normal reference range, T lymphocyte subset

CLC Number:

R446.63

DONG Jingyi, NING Xiaoxiao, WANG Lei, TIAN Jianhui. Investigation on the reference ranges of peripheral blood T lymphocyte subsets among healthy adults in Shanghai[J]. , 2014, 29(6): 617-622.

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