Abstract: Objective To investigate the reference ranges of reticulocyte absolute value(RET#) and percentage(RET%) and its grouping parameters of adults in Shanghai. Methods By XE-2100 automatic hematology analyzer, the reticulocyte and it relative parameters were determined among 2 000 healthy subjects. There were 1 116 males and 884 females. A total of 2mL venous whole blood samples were collected for anticoagulation by ethylene diamine tetraacetic acid-dipotassium (EDTA-K₂). The samples were determined within 2h after collection. Statistical analysis was performed by SPSS11.0 software. Results The reference ranges of RET% were (0.61-1.87)% in males and (0.60-1.81)% in females, respectively. The RET# were (0.030-0.096)×10¹²/L in males and (0.025-0.082)×10¹²/L in females. The retios for low fluorescent reticulocyte(LFR), middle fluorescent reticulocyte(MFR) and high fluorescent reticulocyte(HFR) were (80.8-97.1)%, (2.8-18.1)% and (0.0-2.3)% in males and(82.3-97.9)%, (2.0-15.9)% and (0.0-1.8)% in females, respectively. By classifying the healthy subjects as age, there was no statistical significance in RET# and RET% (P=0.138 and P=0.061). However, there was statistical significance for grouping parameters (P<0.01). Results The reference ranges of reticulocyte parameters are established for adults in Shanghai. The clinical application significance of reticulocyte parameter determination is improved.

Key words: Reticulocyte, Reference range, Automatic hematology analyzer